Silver nanoparticles (Hematite (α-Fe 2 O 3 ) have been used as an antimicrobial and as disinfectant. Nevertheless, there is limited data about its antitumor potential. This study focused on investigating the cytotoxic effects of Hematite (α-Fe 2 O 3 ) from Butea monosperma flower extract on MCF-7 breast cancer cells and its mechanism of action. Green method was created for the synthesis of Hematite (α-Fe 2 O 3 ) using an aqueous extract of B. monosperma flower. Synthesis of hematite (α-Fe 2 O 3 ) was described by different analytical techniques including ultraviolet-visible spectrophotometer, field-emission scanning electron microscopy, X-ray diffraction, and Fourier transforms infrared spectroscopy. Cell viability was determined by the 3-[4, 5-dimethylthiazol-2-yl]-a 2,5-diphenyltetrazolium bromide assay. Reactive oxygen species (ROS) formation was measured using probe 2',7'-dichlorofluorescein diacetate and intracellular calcium (Cai 2+ ) was evaluated with probe flu3-AM. Cells were treated with different concentrations of hematite (α-Fe 2 O 3 ) (1, 3, 6, 10, 15, 25, 50 and 100 μg/mL). The results showed that hematite (α-Fe 2 O 3 ) hindered cell growth in a dose-dependent manner. Hematite (α-Fe 2 O 3 ) appeared to have dose-dependent cytotoxicity against MCF-7 cells through activation of the ROS generation and an increase in the intracellular Cai 2+ (IC 50 52 ± 3.14). In conclusion, the results of this preliminary study demonstrated that hematite (α-Fe 2 O 3 ) from B. monosperma flower extract may be a potential therapeutic potential medicament for human breast cancer treatment.

Among nanoparticles used for medical applications, Camellia Sinensis Nanoparticles (CSNPs) are among the least investigated. This study was undertaken to develop CSNPs by green synthesis using Camellia sinensis tea (Theaceae) plant extract to produce the NPs. The Camellia sinensis, Indian tea plant used from ancient time to increase appetite. Other medicinal uses have also been employed for the synthesis of super paramagnetic α Fe2O3 nanoparticles (NPs). The plant extracts revealed the phenolic groups bifunctional nature and capping nature through the –OH bonding over the nanoparticles (NPs) surface. The prepared nanoparticles (NPs) shows α-Fe2O3 phase among iron oxides and spherical morphology with an average size around 5 nm. The magnetic measurements proved the superparamagnetic behaviour of NPs with non-saturating MS value of 8.5 emu/g at room temperature (300 K). The CSNPs were characterized by UV-V is spectroscopy and X-ray Diffractometry, and evaluated with Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM) and Fourier Transform Infrared (FTIR). The CSNPs were spherical (size 7-20 nm) and contained phenols and flavonoids acquired from the Camellia sinensis extract. CSNPs has good 1-Diphenyl-2-Picrylhydrazyl (DPPH), OH, and NO scavenging properties. MTT assay showed that CSNPs (IC50 = 0.006 μM) were more antiproliferative toward the human MCF-7 cells than the Camellia sinensis tea extract (IC50 = 0.894 μM), Gemcitabin (IC50 = 2.133 μM). The anticancer cell effects of CSNPs on MCF 7 are mediated through the induction of apoptosis and G2/M cell-cycle arrest.

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Background: This study deals with the anti-metastasis and anti-biofilm activities using silver nanoparticles (AgNPs) against human breast cancer cells (MCF-7) via in vitro methods. Different studies have been reported that biofilms formed by Klebsiella pneumoniae, Escherichia coli and Staphylococcus aureus are resistance to most of the recently used antibiotics due to MDR effect. Materials and methods: The characterization of biosynthesized particles was done by UV-vis spectroscopy, FTIR, SEM and XRD techniques. Cytotoxicity analysis was done by MTT assay and Neutral Red Uptake (NRU) assay. Anti-biofilm activity was carried out by using the bacterial strains of Klebsiella pneumoniae, Escherichia coli and Staphylococcus aureus respectively. Results: AgNPs exhibit strong absorption peak in the 420-500 nm range due to Surface Plasmon Resonance in UV-Vis spectroscopy. In FTIR analysis, we found phenol and alcohol compounds (at 3447 cm -1 ), carbonyl groups (at 1631 cm -1 ), alcoholic groups (at 1384 cm -1 ) and polysaccharides (at 1093 cm -1 ). In XRD that AgNPs formed were highly crystalline in nature. In SEM the diameter of AgNPs ranges from 30-90 nm and TEM depicts the size of AgNPs to be 15 ± 2 nm. T. natans-AgNPs exhibits strong cytotoxic activity against MCF-7 breast cancer cell line. Conclusion: The presence of different bioactive molecules in T.natans shows that, green synthesized AgNPs significantly inhibited proliferation of MCF-7 breast cancer cells by inducing apoptosis as well as antibiofilm activity using different bacterial colonies.For the very first time we are reporting the anticancer drugs repositioned as a potential antibiofilm agent in the Klebsiella pneumoniae, Escherichia coli and Staphylococcus aureus organisms.