No abstract

No abstract

Introduction: Traditional medicines for mammary tumour are unreasonable and have genuine symptoms. Non-ordinary normal medications have increased wide acknowledgment because of their assurance of a cure with negligible or no symptoms, however minimal experimental confirmation exists. One such basic cure is the leaves of the Euphorbia hirta plant. Methods: It is initially reported utilization of the fluid concentrate of Euphorbia hirta leaves breast cancer cells. The capacity of the concentrate to impel apoptosis and corruption in the human bosom malignancy cell line MCF-7, contrasted with typical human skin fibroblasts (MDA MB-231), was dictated by morphological changes in the cells utilizing light microscopy, DNA fragmentation, and brilliant stains (Annexin V and Propidium Iodide) utilizing Flow Cytometry and fluorescent microscopy. Results: Apoptosis was instigated in both cells, and more in MCF-7, when they were treated with 25% and half concentrate, while rot was watched mostly after presentation to raised concentrate fixations (75%). DNA discontinuity came about for both cells, in a period and dosage subordinate way. Both cells, at all concentrate fixations, demonstrated no critical contrasts in the quantity of living, dead, apoptotic, and necrotic cells. Conclusion: At long last, the outcomes might show that apoptotic changes in MCF-7 might be free of caspase-3, which is included in apoptosis and is inadequate in MCF-7 cells.

No abstract

Tafethion (ethion), an organophosphate insecticide was examined for its ability to induce chromosomal aberration and micronuclei (MN) in somatic (bone marrow) cells and sperm-shape abnormality in germ cells of mice tested in vivo.Mutagenicity of commercial preparation of ethion (tafethion 50 EC) was tested employing three doses (15, 22.5 and 30 mg/kg b.w.), three routes of adrtrlnistration (ip, po and sc), three hours of exposure acutdy (6, 24 and 48) as well as chronic (6 mg/kgfday; 5 days) for chromosomal aberration. All three doses were also tested for MN (30 hrs) and sperm-shape abnormality (35 days). The negative control was on glass double distilled (g.d.d.) water. The results in general, differed significantly from controls and indicated the mutagenic property of the chemical.