Relative genotoxicity of trichloroacetic acid (TCA) as revealed by different cytogenetic assays: bone marrow chromosome aberration, micronucleus and sperm-head abnormality in the mouse

Bhunya, Sourav; Behera, B.C.

doi:10.1016/0165-1218(87)90092-9

Cited by 34 publications

(4 citation statements)

References 30 publications

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“…Toxicological studies have demonstrated the adverse effect of HAA exposure on the internal structure of testis and epididymis, sperm morphology, and sperm motility parameters. , In humans, several studies also reported associations between HAA exposure and reduced semen quality parameters, including sperm count, concentration, motility, and morphology. , In addition to these traditional semen quality parameters, several laboratory techniques used to evaluate spermatozoa apoptosis and DNA damage are strongly recommended because of their high repeatability and ability to measure different aspects of semen quality . Accumulating evidence from in vivo and in vitro experiments shows that DBPs, including HAAs, can trigger genetic damage and apoptosis. − However, no human study has explored the associations of HAA exposure with spermatozoa apoptosis or DNA damage.…”

Section: Introductionmentioning

confidence: 99%

“…4,5 Given the cytotoxicity, carcinogenicity, genotoxicity, and reproductive toxicity of HAAs, 6,7 the maximum contaminant level (MCL) for TCAA has been regulated to 100 μg/L by the Chinese drinking water standard. 8 Toxicological studies have demonstrated the adverse effect of HAA exposure on the internal structure of testis and epididymis, 6 sperm morphology, 9 and sperm motility parameters. 10,11 In humans, several studies also reported associations between HAA exposure and reduced semen quality parameters, including sperm count, concentration, motility, and morphology.…”

Section: ■ Introductionmentioning

confidence: 99%

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Associations of Urinary Trichloroacetic Acid Concentrations with Spermatozoa Apoptosis and DNA Damage in a Chinese Population

Chen

Liu

et al. 2022

Environ. Sci. Technol.

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Exposure to trichloroacetic acid (TCAA) has been associated with impaired semen quality; however, its association with spermatozoa apoptosis and DNA damage remains unclear. We, therefore, collected single semen and repeated urine samples from male partners of couples attending a reproductive center, which were measured for spermatozoa apoptosis and DNA damage parameters and TCAA concentrations, respectively. Multivariable linear regression models were used to explore the associations between urinary TCAA concentrations and spermatozoa apoptosis (n = 462) and DNA damage parameters (n = 512). After adjusting for potential confounders, positive dose–response relationships were found between urinary TCAA concentrations and percentage of tail DNA (tail%) and tail-distributed moment (TDM) (both p for trend <0.10). Compared with men in the lowest tertile of urinary TCAA concentrations, men in the highest tertile had a greater tail% and TDM of 6.2% (95% CI: 0.7, 12.2%) and 8.9% (95% CI: −1.9, 20.5%), respectively. Urinary TCAA concentrations were unrelated to spermatozoa apoptosis parameters in a dose–response manner. However, urinary TCAA concentrations were positively associated with the percentage of Annexin V+/PI– spermatozoa (apoptotic cells), when urinary TCAA concentrations were modeled as continuous variables. Our results suggest that exposure to TCAA at concentrations in real-world scenarios may be associated with spermatozoa apoptosis and DNA damage.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Associations of Urinary Trichloroacetic Acid Concentrations with Spermatozoa Apoptosis and DNA Damage in a Chinese Population

Chen

Liu

et al. 2022

Environ. Sci. Technol.

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“…Break chromosome,Gap chromosome, Gap chromatid and Deletion. These results may be relating with bacterial products effects upon either protein nature present in chromosomes or DNA molecules [19]. The MN may be produced as an exposure to bacterial products, lead to form chromosome fragments as a result of chromosomes breakage, chromosomes deletion or loss of chromosomes centromers late in anaphase and telophase of cell division, therefore normal chromosomes forming normal nuclei while chromosomes fragmented convoluted around themselves then converted to micronuclei [5].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Study of Mutagenicity and Genotoxicity of Some Bacterial Secretions associated with Bladder Tumors Tissues on Human Lymphocytes by Using Blood Tissue Culture Techniques

Al-Bayati,

Guirges

2011

JFacMedBagdad

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Background: Cytogenetic studies have been used to assess carcinogenic or mutagenic exposures and effects early in occupational, biological and environmental settings. Bacterial infection has not traditionally been considered asa major causes of cancer. Bacteria have been linked to cancer by two mechanisms: induction of chronic inflammation and production of carcinogenic bacterial metabolites.Objective: Because of new concepts in relating infection with certain malignancies the present study is performed. Therefore this study was conducted to determine the effects of bacterial products associated with biopsies of bladder tumor on human lymphocytes tissue cultures in vitro. Moreover, if there is any relation between these products for induction chromosomal aberration (CA) and formations of micronucleus (MN).Subjects and Methods: Chromosomal aberration and micronucleus assay were studied on six identified bacterial isolates of bladder tumor biopsies included 2 isolates of Escherichia coli , 2 isolates of Pseudomonas aeruginosa and 2 isolates of Klebsiella pneumoniae. The bacterial products activity for induction (CA) and (MN) were assayed according to the procedure mentioned in the text.Results: The results showed that each bacterial species have significant effects for induction different types of structural chromosomal aberration (CA) and formation of (MN).Conclusion: K. pneumoniae yielded high significant related predisposing factor in inducing different cytogenetic analysis followed by P. aeruginosa and E. coli .

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“…Mutagens that affect germ cells are also known to have spermatotoxic effects in mice 34 and generally cause an increase in the frequency of sperm head abnormality. 35 Evenson et al 36 demonstrated that such spermatotoxic effect might be due to alteration of testicular DNA and sperm chromatin structure. The feeding of AA could combat the spermatotoxic effects to some extent.…”

Section: Discussionmentioning

confidence: 99%

Protective action of an anti-oxidant (L-Ascorbic acid) against genotoxicity and cytotoxicity in mice during p-DAB-induced hepatocarcinogenesis

Surjyo¹,

Anisur²

2004

Indian J Cancer

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BACKGROUND: DNA damage from micronutrient deficiencies has been suggested as one major cause of cancer.Therefore studies involving vitamin supplementaion , particularly with those with anti-oxidant activity, in combating cancer have routinely been carried out in both in vivo and in vitro systems, but relatively much less in mice. AIMS: The present study examines if L-Ascorbic acid (AA; vitamin C) administration has any protective abilities in combating p-DAB induced hepatocarcinogenesis in mice at cytogenetical, biochemical, histological and ultra-structural levels. SETTINGS AND DESIGN:To test if AA had a protective action against genotoxicity, cytotoxicity and tissue damage in liver during p-dimethylaminoazobenezene (p-DAB) induced hepatocarcinogenesis in mice, a group of mice were chronically fed 0.06% p-DAB and 0.05% phenobarbital (PB) for a varying period of time (7, 15, 30, 60, 90 and 120 days). A sub-group of the p-DAB plus PB fed mice were also fed 1% L-ascorbic acid. Several assays were periodically conducted (at the six intervals of fixation) for determination of genotoxic (based on chromosomal, nuclear and sperm head anomalies), cytotoxic (based on the marker enzymes aspartate transaminase; AST, alanine aminotransferase; ALT; acid phosphatase; ACP; alkaline phosphatase; ALKP; lipid peroxidation; LPO); and tissue damaging (based on optical and electron microscopic studies of liver at day 60 only) effects in these different groups of mice as compared to normal healthy control. METHODS AND MATERIAL: Adult healthy mice of Swiss Albino strain, reared and maintained in the animal house of the Department of Zoology,. Kalyani University, under supervision of Animal Welfare Committee (which oversees ethical issues), served as materials for the present study. Widely practiced standard technique has been followed for each protocol. STATISTICAL ANALYSIS USED:The significance test between different series of data was conducted by student¢s t-test. RESULTS AND CONCLUSIONS: The results of all these studies indicated that AA had protective action against p-DAB induced hepatocarcinogenesis in mice.

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Relative genotoxicity of trichloroacetic acid (TCA) as revealed by different cytogenetic assays: bone marrow chromosome aberration, micronucleus and sperm-head abnormality in the mouse

Cited by 34 publications

References 30 publications

Associations of Urinary Trichloroacetic Acid Concentrations with Spermatozoa Apoptosis and DNA Damage in a Chinese Population

Associations of Urinary Trichloroacetic Acid Concentrations with Spermatozoa Apoptosis and DNA Damage in a Chinese Population

In Vitro Study of Mutagenicity and Genotoxicity of Some Bacterial Secretions associated with Bladder Tumors Tissues on Human Lymphocytes by Using Blood Tissue Culture Techniques

Protective action of an anti-oxidant (L-Ascorbic acid) against genotoxicity and cytotoxicity in mice during p-DAB-induced hepatocarcinogenesis

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