No abstract

P hyTOChemICAl study of the aerial parts of Euphorbia paralias and Euophorbia geniculata belonging to family (euophorbiaceae) grown in egypt was carried out. This study revealed the isolation of eighteen compounds: β-amyrin (1), β-sitosterol and Stigmasterol mixture (2 & 3), Cholesterol (4), Campesterol (5), (Erythradiol, Ovaol and Betulinol mixture) (6-8), 23-acetyl-3-methyl-oleanolic acid (9) and β-Sitosterol-3-O-glucoside (10) were isolated from dichloromethane fraction of E. paralias. While the compounds 1 to 5 and 10 were isolated from E. geniculata. The ethyl acetate fraction of E. paralias l. afforded the isolation of Gallic acid (11), Ellagic acid (12), Querecetin-3-glucopyranoside (13) and Querecetin-3-arabinoside (14), kaempferol-3-(6''-(2'''-galloyl-glucopyranoside) (15). While compounds 11 to 14 in addition to Querecetin-3-rutinoside (rutin) (16), Querecetin-3-rhamnoside (17), and Querecetin (18) were isolated from E. geniculata Ortega. Compound 9 (23-acetyl-3-methyl-oleanolic acid) was isolated for the first time from the nature. Biological activities were carried out including cytotoxic and antiviral and antimicrobial activities of the dichloromethane and ethyl acetate fractions of the aerial parts of E. paralias l. and E. geniculata Ortega revealing significant antimicrobial activity of ethyl acetate fractions of E. paralias l. and E. geniculata Ortega and strong cytotoxic activity of the ethyl acetate fractions in addition to positive antiviral activity of the total extract of E. paralias l.

Euphorbia species contain series of macrocyclic diterpenoids which present as mono, di and sometimes triesters and were reported to possess several and diverse biological activities. The aim herein was to use the ultra-performance coupled to the positive ion mode electron spray ionization mass spectroscopy (UPLC-ESI-MS) as an analytical tool for rapid screening and tentative identification of diterpenoids in the methylene chloride fractions of Euphorbia paralias and Euphorbia geniculata. The identification of compounds was based on ESI-MS and ESI-MS/MS fragmentation pattern, in addition to comparison with reported literature. Five classes were determined including tigliane, ingenane, lathyrane, daphnane and jatrophane diterpenoids esters. Each class of the detected diterpenoids obtained its characteristic ESI-MS/MS fragmentation which facilitates its identification. As a result, a total of fifty-two diterpenoids were identified and characterized in Euphorbia paralias and Euphorbia geniculata. All of these compounds are first report in the two plants by this method. Peak 35 was tentatively identified as ingenol-20-hydroxyheptanoate which expected to be a new compound. The results demonstrated that the used method could be a rapid, and an effective analytical tool for screening and characterization of diterpenoids in the complex systems of plant extracts.