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HIV (human immunodeficiency virus) belong to family of human retroviruses of lentivirus subfamily. This virus hits the patient’s immune system by destroying important cells that fight against disease and infection. Different parameters like Total Leucocytes count TLC, Hb level, platelets count and among male and female were observed.Study of different parameters in HIV (human immunodeficiency virus) patientsfrom different areas of Punjab, Pakistan. Complete blood count (CBC), Hb Level, HIV (human immunodeficiency virus), Platelets count, Total Leucocytes count TLC was done using kits and screening methods.RNA extraction and PCR was done for future analysis.HIV positive males develop anemia and about 9.3% (11) patients are suffering from leukocytosis. 12% of HIV positive males develop thrombocytopenia while 8% have thrombocytosis.