With increasing resolution of microbial diversity at the genomic level, experimental and modeling frameworks that translate such diversity into phenotypes are highly needed. This is particularly important when comparing drug-resistant with drug-sensitive pathogen strains, when anticipating epidemiological implications of microbial diversity, and when designing control measures. Classical approaches quantify differences between microbial strains using the exponential growth model, and typically report a selection coefficient for the relative fitness differential between two strains. The apparent simplicity of such approaches comes with the costs of limiting the range of biological scenarios that can be captured, and biases strain fitness estimates to polarized extremes of competitive exclusion. Here, we propose a mathematical and statistical framework based on the Lotka-Volterra model, that can capture frequency-dependent competition between microbial strains within-host and upon transmission. As a proof-of-concept, the model is applied to a previously-published dataset from in-vivo competitive mixture experiments with influenza strains in ferrets (McCaw et al., 2011). We show that for the same data, our model predicts a scenario of coexistence between strains, and supports a higher bottleneck size in the range of 35-145 virions transmitted from donor to recipient host. Thanks to its simplicity and generality, such framework could be applied to other ecological scenarios of microbial competition, enabling a more complex and nuanced view of possible outcomes between two strains, beyond competitive exclusion.

Arbovirus outbreaks in communities are affected by how vectors, hosts and non-competent species interact. In this study, we investigate how ecological interactions between species and epidemiological processes influence the invasion potential of a vector-borne disease. We use an eco-epidemiological model to explore the basic reproduction number R0 for a range of interaction strengths in key processes, using West Nile virus infection to parameterize the model. We focus our analysis on intra and interspecific competition between vectors and between hosts, as well as competition with non-competent species. We show that such ecological competition has non-linear effects on R0 and can greatly impact invasion risk. The presence of multiple competing vector species results in lower values for R0 while host competition leads to the highest values of risk of disease invasion. These effects can be understood in terms of how the competitive pressures influence the vector-to-host ratio, which has a positive relationship with R0. We also show numerical examples of how vector feeding preferences become more relevant in high competition conditions between hosts. Under certain conditions, non-competent hosts, which can lead to a dilution effect for the pathogen, can have an amplification effect if they compete strongly with the competent hosts, hence facilitating pathogen invasion in the community.

The invasion of vector-borne diseases depends on the type of specific features of the vector and hosts at play. Within the Culex pipiens complex, differences in ecology, biology, and vector competence can influence the risk of West Nile virus (WNV) outbreaks. To determine which life-history traits are most important, we constructed an epidemiological Susceptible-Exposed-Infectious-Recovered model with three vector eco-types, Culex pipiens pipiens, Cx. pip. molestus, and their hybrids, and two vertebrate hosts, birds (as amplifying hosts) and humans (as dead-end hosts). We investigated how differences in feeding preferences and transmission rates influenced WNV transmission across different habitats and two simulated scenarios, i.e., global change and increasing urbanisation settings, to investigate the impact of increasing mosquito and human abundance on the WNV transmission risk. Our results showed that vector feeding preferences and the transmission rate between mosquitoes and birds were the parameters that most impacted WNV invasion risk. Natural habitats were overall more susceptible to WNV invasion, although rural habitats were also susceptible in a climate change scenario. Pipiens-type-related factors dominated virus amplification in natural and rural habitats, while molestus-type-related factors drove the spread of WNV in urban environments. Contrary to common opinion, our findings suggest that hybrids may play only a minor role in WNV transmission. Our study will improve current vector surveillance and control programs by targeting specific vector types in specific environments, especially in rural settings, which are most responsive to environmental shifts. This approach can help reduce wasted time and economic costs while maximizing the efficiency of local public health authorities.