Background: Pertolongan persalinan merupakan faktor penting dalam upaya menurunkan angka kematian ibu dan anak. Di Indonesia pemanfaatan pertolongan persalinan oleh tenaga kesehatan yang kompeten masih kurang dibandingkan target yang diharapkan, termasuk di Kecamatan Klabang Kabupaten Bondowoso pada tahun 2018 hanya mencapai 86,10%.Objectives: Penelitian ini bertujuan untuk menganalisis pengaruh penyuluhan berbasis video whatsapp tentang persalinan terhadap pengetahuan dan sikap ibu hamil trimester III di Puskesmas Klabang Kabupaten Bondowoso.Design: Penelitian ini menggunakan desain One Group Pretest-Posttest. Populasi penelitian ini adalah semua ibu hamil Trimester III di wilayah kerja Puskesmas Klabang yang diperkirakan melahirkan pada bulan Mei-Juni tahun 2019, dengan jumlah sampel sebesar 33 orang. Analisis data menggunakan uji Wilcoxon dengan nilai signifikansi sebelum dan setelah penyuluhan sebesar 0,000 < 0,005.Results: dari 33 responden, 20 responden menunjukkan adanya peningkatan sikap yang positif setelah diberikan eksperimen. Hasil analisa statistik dengan uji Wilcoxon diperoleh nilai p- value 0,000 (p<0,05), artinya terdapat pengaruhartinya terdapat perbedaan sikap sebelum dan sesudah dilakukan penyuluhan berbasis video whatsapp tentang persalinan. Sehingga H0 ditolak yang berarti bahwa ada perbedaan atau hipotesa peneliti diterima.Conclusions: Disimpulkan bahwa ada pengaruh penyuluhan berbasis video whatsapp tentang persalinan terhadap pengetahuan dan sikap ibu hamil trimester III di Puskesmas Klabang Kabupaten Bondowoso.

ABSTRAK Pertolongan persalinan merupakan faktor penting dalam upaya menurunkan angka kematian ibu dan bayi. Di Indonesia pemanfaatan pertolongan persalinan oleh tenaga kesehatan yang kompeten masih kurang dibandingkan dengan target yang diharapkan, salah satunya di Kecamatan Klabang Kabupaten Bondowoso pada tahun 2018 hanya mencapai 86,10%. Penelitian ini bertujuan untuk menganalisis pengaruh penyuluhan berbasis video whatsapp tentang persalinan terhadap pengetahuan dan sikap ibu hamil trimester III di Puskesmas Klabang Kabupaten Bondowoso. Penelitian ini menggunakan desain One Group Pretest-Posttest. Populasi pada penelitian ini yaitu semua ibu hamil Trimester III di wilayah kerja Puskesmas Klabang yang diperkirakan melahirkan pada bulan Mei-Juni tahun 2019, dengan jumlah sampel sebesar 33 orang. Analisis data menggunakan uji Wilcoxon dengan nilai signifikansi sebelum dan setelah penyuluhan sebesar 0,000 < 0,005. Terdapat pengaruh penyuluhan berbasis video whatsapp tentang persalinan terhadap pengetahuan dan sikap ibu hamil trimester III di Puskesmas Klabang Kabupaten Bondowoso. Kata Kunci : Persalinan, Penyuluhan Kesehatan, Video WHATSAPP ABSTRACT Childbirth assistance is an important factor in efforts to reduce maternal and infant mortality. In Indonesia the use of childbirth assistance by competent health workers is still below the expected target, including in Klabang Subdistrict, Bondowoso Regency in 2018 only reach 86.10%. This study aims to analyze the effect of whatsapp video-based counseling about childbirth on the knowledge and attitudes of thirdmester pregnant women in the Puskesmas Klabang, Bondowoso Regency. This study used a One Group Pretest-Posttest design. The population of this study were all thirdmester pregnant women in the working area of the Klabang Community Health Center who were expected to give birth in May-June 2019, with a total sample of 33 people. Data analysis used the Wilcoxon test with a significance value before and after counseling of 0,000 <0.005. There was an influence of whatsapp video-based counseling about childbirth on the knowledge and attitudes of trimester III pregnant women in the Puskesmas Klabang, Bondowoso Regency.

BACKGROUND: Cholestasis caused by obstruction of the common bile duct and may developed gut-derived sepsis due to reactive oxygen species (ROS) accumulation. Ursodeoxycholic acid (UDCA) and glutathione are widely known for their antioxidant properties, that might be beneficial against ROS. However, the effects of UDCA-glutathione combination against ROS have not been well elucidated in previous studies. Thus, this study was conducted to evaluate tumor necrosis factor (TNF)-α level and height of terminal ileal mucosal villus after UDCA-glutathione administration in cholestasis rat model.METHODS: Twenty-eight male Sprague Dawley rats were randomly grouped into four treatment groups, each group consisted of seven rats that had previously undergone bile duct ligation. Three groups received treatment of UDCA-glutathione combination on stratified dose, while the other one only received UDCA. Each treatment was given for 21 days. Ileal samples were collected from the rats and stained with mouse anti TNF-a antibody and hematoxylin-eosin (HE). Immunohistochemistry and histopathological examination were done using microscope and then calculated with ImageJ.RESULTS: The combination of UDCA and glutathione treatment decreased the TNF-α expression (p<0.05) compared to UDCA only group, particularly in group that received 20 mg UDCA and 15 mg glutathione supplementation (p<0.05) and group that received 30 mg UDCA and 20 mg glutathione supplementation (p<0.05). The height of the mucosa villous was higher in the UDCA-glutathione combination groups for all the three dosage variations given (p<0.05) compared to UDCA only group.CONCLUSION: UDCA-glutathione combination downregulates TNF-α expression and improves ileum mucosal villus height in cholestasis.KEYWORDS: cholestasis, glutathione, intestinal villus height, TNF-α, UDCA

Background Peritonitis is the second most common cause of severe sepsis that associated with a significant mortality rate. Due to a large gap of newer antibiotics innovation and antibiotic resistance emergence, the use of antioxidant has a possible alternative as adjuvant therapy in peritonitis management. It has been studied that glutathione as an alternative in the development of new anti-inflammatory effect. Thus, the aim of this study was to evaluate the levels of TNF-α and IL-10 after glutathione administration as adjuvant therapy in rat peritonitis model. Materials and methods Male wistar rats were divided into four groups (n = 6 per group), Group 1: control group (C), Group 2: peritonitis group (P), Group 3: peritonitis + Ceftriaxone group (P + Cef), Group 4: peritonitis + Ceftriaxone + Glutathione group (P + Cef + Glu). Twenty-four hours after peritonitis induction, the blood samples were taken to evaluate TNF-α and IL-10 levels. Results There was a significantly increase of mean TNF-α level in group 2 (P) 473,86 ± 388,99 pg/ml (p value 0,00) and significantly decrease of mean TNF-α level after glutathione injection in group 4 (P + Cef + Glu) (p value 0,02). No significant changes in IL-10 levels in rats peritonitis model. Conclusions Glutathione supplementation is significantly decrease the mean level of TNF-α in rats induced peritonitis, however there is no difference compare to antibiotic only. Moreover, there no significant changes level of IL-10 in rats induced peritonitis after glutathione injection.