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Pangasius catfish are not able to synthesize amino acids in their bodies so that Pangasius catfish need a supply of amino acids such as lysine in their feed. This study aims to find out the effect of lysine addition in commercial feed towards the growth, protein content, and fatty acid of Pangasius catfish meat. Total doses of lysine added to the commercial feed are: P0 (0%), P1 (1.2%), P2 (2.2%), dan P3 (3.2%). The results showed that the addition of lysine essential amino acids in commercial feed showed a significantly different effect (P<0.05) on t h e specific growth rate, feed efficiency, feed conversion ratio, Saturated Fatty Acid (SFA), and Monounsaturated Fatty Acid (MUFA) of Pangasius catfish meat. On the other hand, Polyunsaturated Fatty Acid (PUFA) showed a very significant difference (P<0.01) with lysine addition dose of 3.2% and no significant effect (P>0.05) on SR. The results of this study showed that lysine addition dose of 1.2% could increase SGR, feed efficiency, and FCR, and lysine addition dose of 2.2% can increase the protein content of Pangasius catfish meat also lysine addition dose of 3.2% provides the best performance for fatty acids of Pangasius catfish meat.

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Grouper is one of the fish that is much sought after by the general public because grouper have high economic value and one of the exported fish products. The high demand for grouper to cause community develop hatcheries grouper. Appropriate feeding and efficiency very influential towards the process of optimal fish growth. One of the feeding management of frequently used IE frequency feeding. Feed frequency is influenced by several factors, namely age, type and the appetite of the fish itself. The purpose of this study is to see the number of frequency of feeding on seeds of hybrid grouper cantang best to promote growth and feed efficiency and to lower the value of the feed conversion. This study used a RCD. The results showed that the granting of different frequency-feed can increase the growth of specific daily on the seed of hybrid cantang grouper. The highest growth rate of treatment weight i.e. P2 of 4.2 %/day. The value of the efficiency of feed conversion ratio and best i.e. P1 of 67% and 1.55. Treatment of the P1 value is the SGR. efficiency of feed conversion ratio and the lowest i.e. respectively 3.3%/day. 46% and 2.26.

The research aimed to find out whether there was interaction between fermentation duration and probiotics dose toward the increase in crude protein and decrease in crude fiber content in coffee peel. This research was experimental using complete randomized design (RAL) factorial, data analysis using duncan. The result of the research showed that there was interaction between duration and probiotics doses in coffee peel fermentation to crude protein in day 5 of fermentation duration with probiotics dose of 5%, it was the highest result of crude protein content. There was no interaction between duration fermentation and probiotics dose in fermentation of coffee peel toward crude fiber content in day 7 duration of fermentation with probiotics dose of 5%, it was the result of the lowest crude fiber. Fermentation in 7 days with dose of 5% was the best result. There was increase of protein from 12.15% to 13.93% and decrease of fiber from 34.27% to 30.8%.