2-D transient seepage beneath a dam was investigated by the finite element method. Governing equations were considered in coupled and uncoupled methods. At first stage, the fluid continuity equation for compressible porous media considered as uncoupled. Because of the occurrence of seepage forces, and their interaction with the fluid pore pressure, particularly in high compressible soils, uncoupled methods seem to be far from real conditions. Therefore force equilibrium equations were taken into account by coupling with the fluid continuity equation. Finite element formulation based on Galerkin method. Results of two mentioned methods were compared with steady-state seepage results. Comparing the results of coupled and uncoupled models showed that the time require to reach steady-state condition in coupled model considerably shorter than the uncoupled model. Coupled analyses shows that the effective stress due to seepage forces were smaller than ones calculated by the uncoupled method
Aims
Microbial induced calcium carbonate precipitation (MICP) is one of the bio‐cementation methods for improving granular soils. This study evaluate the feasibility of obtaining a bacterial solution with high optical density and urease activity by an inexpensive corn steep liquor (CSL) medium in non‐sterile conditions in order to achieve sand improvement.
Methods and Results
Corn steep liquor media with different concentrations (different dilution rates) were prepared and, without any autoclaving (non‐sterile conditions), different percentage of the inoculum solutions were added to them and incubated. Effect of inoculum solution percentage and CSL dilution rates on specifications of bacterial solution was evaluated. Urease activity and scanning electron microscope (SEM) and X‐Ray Diffraction (XRD) were used to efficiency of CLS media in sand improvement. The considerable urease activity was measured as 5·7 mS cm−1 min−1 using nonsterile CLS. By using CYNU (CSL‐Yeast extract‐NH4Cl‐Urea) bacterial solution, the urease activity of 5·5 mS cm−1 min−1 for the OD600 (optical density at 600 nm) of 1·88 and, consequently, specific urease activity of 2·93 mS cm−1 min−1 OD600−1 was obtained. The highest unconfined compressive strength (811 kPa) was obtained for the CYNU. XRD revealed new calcite peaks next to the quartz peaks.
Conclusions
Production of inexpensive bacterial solution using diluted CSL as the inexpensive, effective and powerful culture media for Sporosarcina pasteurii cultivation in nonsterile conditions, allows geotechnical and biotechnological engineers to use MICP technology more widely in land improvement and field‐scale bio‐cementation and bioremediation projects.
Significance and Impact of the Study
Obtaining high urease activity of inexpensive microbial solution using diluted CSL as the culture medium in nonsterile conditions, as the unique results of this study, can be significant in the field of bioremediation studies using MICP.
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