2020
DOI: 10.1111/jam.14866
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Non‐sterile corn steep liquor a novel, cost effective and powerful culture media for Sporosarcina pasteurii cultivation for sand improvement

Abstract: Aims Microbial induced calcium carbonate precipitation (MICP) is one of the bio‐cementation methods for improving granular soils. This study evaluate the feasibility of obtaining a bacterial solution with high optical density and urease activity by an inexpensive corn steep liquor (CSL) medium in non‐sterile conditions in order to achieve sand improvement. Methods and Results Corn steep liquor media with different concentrations (different dilution rates) were prepared and, without any autoclaving (non‐sterile… Show more

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Cited by 20 publications
(8 citation statements)
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“…Finally, for future large‐scale field applications, the effectiveness of the culture and enrichment and the cost and environmental risks should be considered carefully. Some alternative inexpensive industrial substrates such as corn steep liquor, vegemite, torula, lactose mother liquor and food‐grade yeast extract that can produce a considerable urease activity have been reported to replace the expensive laboratory‐grade yeast extract (Achal et al, 2009; Chaparro‐Acuña et al, 2018; Joshi et al, 2018; Omoregie et al, 2019; Babakhani et al, 2020). Moreover, some industrial wastes can also be used as the nutrients such as chicken manure effluent (Yoosathaporn et al, 2016).…”
Section: Materials For Micpmentioning
confidence: 99%
“…Finally, for future large‐scale field applications, the effectiveness of the culture and enrichment and the cost and environmental risks should be considered carefully. Some alternative inexpensive industrial substrates such as corn steep liquor, vegemite, torula, lactose mother liquor and food‐grade yeast extract that can produce a considerable urease activity have been reported to replace the expensive laboratory‐grade yeast extract (Achal et al, 2009; Chaparro‐Acuña et al, 2018; Joshi et al, 2018; Omoregie et al, 2019; Babakhani et al, 2020). Moreover, some industrial wastes can also be used as the nutrients such as chicken manure effluent (Yoosathaporn et al, 2016).…”
Section: Materials For Micpmentioning
confidence: 99%
“…Additionally, the cost of the extraction and purification of sugars from steeping water limits its effective utilization. Moreover, the CSW can be combined with gluten, fibrous materials, and solids to use as animal feed [18,19], or to be used as a cost-effective nitrogenous source for microbiological purposes instead of a high-cost one [20,21]. In previous studies, CSW has been used as nitrogen source supplementation for LA [6,22], enzymes [23], and ethanol production [24] at low concentrations.…”
Section: Introductionmentioning
confidence: 99%
“…Corn steep liquor is a by-product of the wet milling process and contains crude proteins, amino acids, vitamins, and other nutrients [ 29 ]. It has been employed as an inexpensive source of nitrogen for microorganisms in the production of enzymes, antibiotics, and other fermentation products [ 30 ]. Different concentrations of corn steep liquor (0, 1, 2, 3, 4 and 5 g/L) were used to investigate the effects of corn steep liquor on γ-PGA production.…”
Section: Resultsmentioning
confidence: 99%