Pasteurella multocida is the common cause of Duck septicemia (Pasteurellosis) which affects ducks leading to high economic losses to duck producers. Pasteurella multocida infections are associated with severe, life-threatening systemic disease involving both hemorrhagic pneumonia and septicemia. A cross-sectional study was carried out on 220 samples collected from different breeds of ducks (Mallard, Muscovy and Baladi) from Minufiya governorate, Egypt. Only 16 cases were positive for Pasteurella multocida infection (7.3%). These isolates were confirmed microscopically, biochemically and rapidly by using Vitek2 compact system. The application of PMT-ELISA and mouse lethality test for the 16 isolates of P.multocida serotypes for differentiation between toxigenic and non toxigenic isolates. All tested of 16 isolates showed to be toxigenic by using PMT-ELISA and mouse lethality test. The application of PCR and multiplex PCR for the detection of tox A gene and capsular serotyping of toxigenic isolates of P.multocida respectively. All 16 P.multocida isolates were positive to tox A and capsular type A (100%). This study concluded that P. multocida serve as a major cause of Duck septicemia (Pasteurellosis) which affects ducks leading to high economic losses in poultry industry. Also indicated that the majority of P. multocida serotypes are toxigenic.

The gastrointestinal tract's most commonly occurring primary mesenchymal tumor is the gastrointestinal stromal tumor (GIST). However, few cases worldwide were reported associated with renal cell carcinoma (RCC). Therefore, we aimed to identify the association of genitourinary tumors in patients with GIST in our tertiary care hospital in Saudi Arabia and compare it to the literature. We identified all patients in the pathology department database with the diagnosis of GIST. We excluded duplicate and recurrent cases. We examined patients’ files for the presence of RCC, adrenal tumors, or other genitourinary cancer. A systematic review of the association was conducted. From 2003 to 2020, 170 patients had a histopathologic diagnosis of primary GIST, 100 men and 70 women, median age of 57 (range 9–91) years at the time of diagnosis. The site of primary GIST was gastric 103, small bowel 43, mesenteric 5, omentum/peritoneum 7, abdomen 4, isolated adrenal 1, and other 7. Six patients had associated primary genitourinary cancer. Three patients had RCC (two clear cell RCC and one radiologic diagnosis only), and three had adrenal tumors (one adrenal carcinoma, one an isolated adrenal GIST, and one pheochromocytoma). In addition, two patients had a tumor invading the urinary bladder. Although the cohort included 63 men aged 60 or above (median 71 ± 8.7 years, range 60–94), none demonstrated clinical prostatic carcinoma. Data was compared to 69 systematic review articles. We report the rare association between GIST tumors and primary genitourinary cancer, mainly RCC and adrenal tumors. Also, we identified a secondary invasion of the urinary bladder. Unlike the reported series, none of the older male patients had clinical prostate cancer.

The authors have withdrawn this preprint due to author disagreement.

BackgroundElimination of most of Egyptian environmental pollution depends on prevention of burning of agricultural wastes specially rice straw. Microbial improvement of nutritive value for rice straw is not only aids in the prevention of Egyptian environmental pollution but also solve the problem of shortage in animal feeds ingredients. The current study was designed to investigate the microbial improvement of rice straw via solid state fermentation using five strains of fungi namely Trichoderma viride, Trichoderma reesei, pleurotus ostreatus, Aspergillus oryzae and Aspergillus Fetidus. ResultsThis fermentation results in improving of nutritive value of rice straw by increasing its dry matter, protein, fat, ash and energy content with decreasing of fiber and organic matter content. These effects were variable according the type of fungi, which were very high for rice straw treated with Trichoderma viride and Trichoderma reesei followed by that treated by pleurotus ostreatus then those treated by Aspergillus oryzae and Aspergillus Fetidus.ConclusionIt could be concluded that the microbial treatment is a good way to improve the nutritional value of rice strawTrial registrationProject Nr.9/2017- Faculty of Veterinary Medicine- University of Sadat City. http://usc.edu.eg/ar/news/13741.aspx

Salmonella is one of the most important causative agents of food poisoning and gastroenteritis in humans. This study spot highlights on isolation, identification and molecular characterization of salmonella serovars from imported frozen meat using the convential and modern molecular tools. Methods: A cross-sectional study was carried out on 100 samples of frozen meat collected from different supermarkets from Minufiya governorate, Egypt. Results: The prevalence of Salmonella were 6%. Serotyping of the obtained salmonella isolated revealed that Salmonella enteritidis, Salmonella typhimurium and Salmonella .paratyphi were the prevalent serotypes in the examined samples. S. typhimurium, only 3 samples (3%) ,Salmonella enteritidis was isolated from only 2 sample (2%) and S.paratyphi only 1 samples (1%). The application of conventional PCR for the six obtained isolates of Salmonella serotypes using universal gene (invA) was effective tool for identification and genotypic of pathogenic Salmonella serotypes. Conclusions: This study concluded that Salmonella is among the most important food borne pathogens worldwide contaminating a wide range of animal products including meat products. Also indicated that the cPCR was specific and rapid method for identification and genotyping of pathogenic salmonella serotypes.