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Acinetobacter species other than Acinetobacter baumannii have rarely been reported to be associated with nosocomial outbreaks of bloodstream infections. Within a period of 1 week, seven Acinetobacter-like isolates were recovered from peripheral blood and catheter specimens of five patients at a neonatal intensive care unit (NICU) in a tertiary hospital in Turkey. All five patients had placement of central venous catheters and had received total parenteral nutrition before the onset of bacteremia. Two of the five patients died. Medical devices, tap water, aerators, water samples, various surfaces, intravenous fluids, and the hands of health care workers in the NICU were sampled and were culture negative for the bacterium. All seven of the isolates had identical biochemical reactions, antimicrobial susceptibility results, and pulsed-field gel electrophoresis patterns, indicating a clonal nosocomial outbreak. A panel of standard biochemical reaction profiles and three phenotypic commercial identification systems failed to identify these isolates. Phenotypically, the isolate differed from Acinetobacter ursingii by its hemolysis on sheep blood agar and its negative citrate utilization. Sequences of the full 16S rRNA gene, which contained at least three different gene copies with polymorphic sequences between nucleotide positions 70 and 206, were determined from the first recovered isolate. The complete 1,529-to 1,531-bp 16S rRNA gene sequences and partial 801-bp rpoB gene sequences had similarities of 99.5% and 97.2%, respectively, to an A. ursingii isolate. The DNA-DNA similarities of the strain against the type strain of A. ursingii were 64.7 and 68.7%, which were lower than the recommended threshold value of 70% for the definition of bacterial species. These data indicate that a novel Acinetobacter organism caused the nosocomial outbreak of bacteremia in the NICU unit. We propose the designation of Acinetobacter septicus sp. nov. for these isolates, with isolate AK001 as the type strain.

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Background: An effectively working health system is not possible without a satisfied workforce. Each year many dis-satisfied professionals either quit their profession or leave jobs in search of better opportunities. This is why the subject of job satisfaction has gained attention in the public health care sector and human resources in Pakistan in the recent past. This particular study was done to assess the job satisfaction of healthcare employees in the public tertiary hospital to identify the various underlying factors. Methods: The data was collected using Job Satisfaction Survey (JSS) questionnaire & analyzed using SPSS18. Results and Discussion: The results of the study showed that majority of the employees were satisfied with their supervisors, nature of job and colleagues but showed dissatisfaction for the rest of the factors like salaries, benefits, communication and conditions at work. Conclusion: If we want to improve the quality of health services that are provided to the consumers of health system i.e. patients then we cannot ignore the significance of satisfied health workforce.