House fly “Musca domestica” Linnaeus is a common insect widely distributed all over the world and is one of the domestic insect pests found associated with human and animal. Due to their habits and habitats, house flies are able to transmit several pathogenic microorganisms to man such as: bacteria, fungi and virus. House flies are not just annoying human and animal, but they also have been known as vectors of infectious microorganisms either mechanically or biologically. Chemical insecticides have been used for many years and have been known as the most effective approach in house fly management but due to their side effects on the environment and the increasing development of pest resistance to each new chemical, studies tended to explore new alternative methods in pest control. Biological methods including different predators, parasites, entomopathogenic micro-organisms and botanical extracts showed in the last years a practical and effective ecofriendly method to control insect pests including house fly and at the same time safe on human and animal.

Abstract. El-Ghwas DE, Al-Nasser AS, Al-Sheikhy AA. 2021. Short Communication: Bacillus endolithicus and Bacillus paramycoides: New Isolates from housefly Musca domestica in Saudi Arabia. Biodiversitas 22: 4209-4214. Housefly Musca domestica Linnaeus is a common insect widely distributed all over the world. It is one of the domestic insects found associated with humans and animals. The present study investigated the bacterial diversity associated with Musca domestica samples collected from different places such as food courts and trash cans near fast-food restaurants in Makkah Province from October 2019 to December 2019. Eighteen pure isolates of bacterial strains were isolated and identified by Gram staining. Most of the bacterial isolates were Gram-positive except for two species, which were Gram-negative. The VITEK system was used to identify randomly isolate no. 5, 7, 17, 18, and 29. The results revealed that they belonged to the genus Bacillus sp., Staphylococcus sp., Pseudomonas sp., and Micrococcus sp. respectively. Due to the most isolated strains were Bacillus sp., so 16S RNA was used to genetically identify novel isolated strains 5 and 7. Isolate no. 5 showed the highest similarity (99 %) with Bacillus endolithicus, and isolate no. 7 showed the highest similarity (99 %) with Bacillus paramycoides. This is the first record of Bacillus endolithicus and Bacillus paramycoides to be isolated from the house fly Musca domestica L.