The present study was aimed to screen the phytochemicals and quantification of alkaloids, phenolic compounds and flavonoids and to evaluate the nematicidal activity of ethanolic leaf extracts of Datura metel, Datura innoxia and Brugmansia suaveolens against Meloidogyne incognita. Phytochemical screening of leaf extracts of D. metel, D. innoxia and B. suaveolens was carried out by qualitative analysis and the results revealed that the presence of bioactive compounds like alkaloids, steroids, flavonoids, terpenoids, phenolic compounds, tannins, anthroquinone glycosides,