Nepal is a part of the world's biodiversity hotspot and ranks the 49 th in the world for biodiversity. Agrobiodiversity and its conservation status were studied through literature review, field survey, key informant survey and focus group discussion. Results of field implementation of some good practices and action research were also documented. Among 24,300 total species in the country, 28% are agricultural genetic resources (AGRs), termed as agrobiodiversity. Agrobiodiversity has six components (crops, forages, livestock, aquatic, insects and microorganisms) and four sub-components (domesticated, semi-domesticated, wild relatives and wild edible) in Nepal. Agrobiodiversity on each component exists at agroecosystem, species, variety/breed/biotype/race/strain, genotype and allele levels, within an altitude range from 60 to 5,000 masl. There are 12 agroecosystems supporting 1026 species under crop component, 510 under forage, 35 under livestock, 250 under the aquatic animal, 17 under aquatic plant, 3,500 under insect and 800 under microorganism. An estimated loss of agrobiodiversity is 40%, however, farmers have reported up to 100% loss of AGRs in some areas for a particular species. Conservation of agrobiodiversity has been initiated since 1986. Four strategies namely ex-situ, on-farm, in-situ and breeding have been adopted for conservation and sustainable utilization of AGRs. Eighty good practices including process, methods and actions for managing agrobiodiversity have been in practice and these practices come under five conservation components (sensitization, method and approach, accelerator, value and enabling environment). Within the country, 18,765 accessions of AGRs have been conserved in different kinds of banks. A total of 24,683 accessions of Nepalese crops, forages and microbes have been conserved in different international and foreign genebanks. Some collections are conserved as safety duplication and safety backup in different CGIARs' banks and World Seed Vault, Korea. Two global databases (GENESYS and EURISCO) have maintained 19,200 Nepalese accessions. Geographical Information System, Climate Analog Tool and biotechnological tools have been applied for better managing AGRs. Many stakeholders need to further concentrate on the conservation and utilization of AGRs. Global marketing of some native AGRs is necessary for sustaining agriculture and attracting young generations as well as conserving them through use.

Garden pea (Pisum sativum L) is an important winter legume used as fresh vegetables and other drier food products. Despite of its importance as cash crop in many parts of Nepal, much study on various aspects for enhancing production and productivity has yet to be done. Therefore, to evaluate the production performance different genotypes of garden pea in eastern hills agro-ecological conditions present experiments were carried out consecutively for two years (2015 and 2016) at Agricultural Research Station, Pakhribas. The experiment comprised of 11 different genotypes of garden pea including a check variety Arkel. The production performance was evaluated in a completely randomized block design with three replications. The seeds were sown at 50 × 10 cm spacing during first week of October for two years. The result showed that DGP-05 genotype had earliest 104 days after sowing. The DGP-08 genotype showed 13 which were the maximum numbers of pods per plant (13), while DGP-01 showed 8 numbers of seeds as the maximum per pod. The DGP-03 genotype had the longest pod of 9.78 cm among others. The highest fresh pod yield of 18.14 t/ha was achieved from genotype DGP-09 followed by Arkel with (16.32 t/ha).

Naked barley (Hordeum vulgare var. nudum L.) is a traditional, culturally important, climate-resilient winter cereal crop of Nepal. Evaluation of the naked barely genotypes for yield and disease is fundamental for their efficient utilization in plant breeding schemes and effective conservation programs. Therefore, to identify high yielding and yellow rust resistant landraces of naked barley for hilly and mountainous agro-ecosystem, twenty naked barley landraces collected from different locations of Nepal, were evaluated in randomized complete block design (RCBD) with three replications during winter season of 2016 and 2017 at Khumaltar, Lalitpur, Nepal. Combined analysis of variances revealed that NGRC04902 (3.46 t/ha), NGRC00886 (3.28 t/ha), NGRC02309 (3.21 t/ha) and NGRC06026 (3.10 t/ha) were the high yielding landraces and statistically at par with the released variety 'Solu Uwa' (3.15 t/ha). The landraces namely NGRC00837 (ACI Value: 1.86) was found resistant to yellow rust diseases. Landraces NGRC06034 (131.7 days) and NGRC02363 (130.8 days) were found early maturing and NGRC02306 (94.36 cm) was found dwarf landraces among tested genotypes. These landraces having higher yield and better resistance to yellow rust need to be deployed to farmers' field to diversify the varietal options and used in resistant breeding program to improve the productivity of naked barley for Nepalese farmers.

An in-vitro experiment was carried out for testing the efficacy of various fungicides by adopting poisoned bait method against Rhizoctonia solani (isolated from large cardamom) in Agricultural Research Station, Pakhribas. The experiments were conducted with Completely Randomized Design (CRD) during 2016 with four replications. Two level of concentration of the fungicides (10 ppm and 100 ppm) were used and concentration was calculated based on active ingredients (a.i.) of the pesticides. At lower concentration (10 ppm), Nativo (Tubeconazole 50% + Trifloxystrobin 25 WG) and Dhanustan (Carbendazim 50 % WP) whereas at higher concentration (100 ppm), Folicure (Tubeconazole 25.9 %) and Saaf (Carbendazim 12 % + Mancozeb 63 % WP) found effective for the inhibitions of mycelia growth of R. solani. For the control of R. solani, Nativo and Dhanustan showed greatest inhibition of mycelia growth at 10 ppm concentration as compared to other fungicides. The fungicides containing Tubeconazole and Carbendazim as active ingredients, shows highest degree of mycelia inhibitions as compared to others. The fungicides found effective for inhibition of mycelia growth of R. solani in this study should be further tested in field conditions to verify their efficacy as well as to determine their optimum doses of application. Int. J. Appl. Sci. Biotechnol. Vol 8(1): 61-64

Capsicum (Solanaceae), comprising of sweet and hot chilli pepper, is a globally known spice crop. This genus is well known for its huge genetic diversity at intra- and inter-species level. Diversity among Akabarechilli landraces, a unique Capsicum from Nepal, has not been studied so far. The present study characterized thirty accessions of Akabarechilli using 26 qualitative and 21 quantitative agro-morphological markers at the experimental plots of National Genebank, Khumaltar, Nepal during the summer season of 2018/019. Using principal component analysis, the first- principal components with eigen-values more than 1 contributed 25% of the variability among accessions for quantitative traits, whereas the first principal components with eigen-values more than 1 contributed 22.2% of the variability among accessions for qualitative traits. These findings suggested a wide range of morphological variations among the tested accessions. Based on qualitative and quantitative traits, 30 accessions were grouped into 6 distinct clusters by Euclidian distance and average method. Accessions CO-11048 and CO-11050 under cluster-III were round shape fruit type and accessions CO-11044, CO- 11046 andCO-11047 under cluster-I were high fruiting with longer fruitbearing period and could be utilized for breeding purpose as these are the traits preferred by consumers or processors in Nepalese market. SAARC J. Agric., 19(2): 37-55 (2021)