Summary Complete and highly accurate reference genomes and gene annotations are indispensable for basic biological research and trait improvement of woody tree species. In this study, we integrated single‐molecule sequencing and high‐throughput chromosome conformation capture techniques to produce a high‐quality and long‐range contiguity chromosome‐scale genome assembly of the soft‐seeded pomegranate cultivar ‘Tunisia’. The genome covers 320.31 Mb (scaffold N50 = 39.96 Mb; contig N50 = 4.49 Mb) and includes 33 594 protein‐coding genes. We also resequenced 26 pomegranate varieties that varied regarding seed hardness. Comparative genomic analyses revealed many genetic differences between soft‐ and hard‐seeded pomegranate varieties. A set of selective loci containing SUC8‐like, SUC6, FoxO and MAPK were identified by the selective sweep analysis between hard‐ and soft‐seeded populations. An exceptionally large selective region (26.2 Mb) was identified on chromosome 1. Our assembled pomegranate genome is more complete than other currently available genome assemblies. Our results indicate that genomic variations and selective genes may have contributed to the genetic divergence between soft‐ and hard‐seeded pomegranate varieties.
The data envelopment analysis (DEA) approach was used to estimate technical efficiency scores which were then regressed against farm specific characters using Tobit regression. Primary data were collected from a random sample of 240 organic and conventional coffee famers (120 from each type). Mean technical efficiency scores were 0.89 and 0.83 for organic and conventional coffee farming, respectively. Scores for farms operating under constant return to scale (CRS), decreasing return to scale (DRS) and increasing return to scale (IRS) were 31.67, 3.83, and 37.5 %, respectively, for organic coffee and 29.17, 25, and 45.83 %, respectively, in conventional farming areas. These scale characteristics define a production set that is closed and convex with property of strong disposability. Tobit regression showed that the variation in technical efficiency was related to education, farm experience and training/extension services, and access to credit. Farmers would reconsider the rationing of input and learn from technically efficient farm practices. Policy implications will rest on production planning strategies. The objective of this study was to estimate the technical efficiency of both types of farming and evaluating the factors affecting farm inefficiency of each farming category of coffee in rural region of Nepal.
The total of thirteen different avocado (Persea americana Mill.) genotypes were collected for evaluating the fruit characteristics in the laboratory of Agricultural Research Station, Pakhribas during November 2017. The fully matured fruits from the farmer's field at Patle, Dhankuta were collected. The criteria for selecting the genotypes were fruit weight, fruit length, fruit diameter, seed weight, pulp weight, pulp to fruit ratio and the seed weight percentage. The result suggests the potentiality of the genotypes PAKAV002 and PAKAV010 in most of the evaluated characters as compared to the tested genotypes. The genotypes PAKAV008 and PAKAV007 were disliked as they have 32.59% and 28.39% of seed weight to the total fruit weight. The genotypes PAKAV002 and PAKAV010 had the average fruit weight ranging (307.1 g and 346.8 g), maximum of pulp to fruit ratio 62.34% and 56.97%. Similarly, genotypes PAKAV010 (11.425%), PAKAV013 (11.96%) and PAKAV002 (14.47%) had low seed weight to the total fruit weight which is regarded important factor for avocado selection and evaluation. This result shows that the genotypes PAKAV002 and PAKAV010 should be further evaluated for fruit characteristics and the quality.
Garden pea (Pisum sativum L) is an important winter legume used as fresh vegetables and other drier food products. Despite of its importance as cash crop in many parts of Nepal, much study on various aspects for enhancing production and productivity has yet to be done. Therefore, to evaluate the production performance different genotypes of garden pea in eastern hills agro-ecological conditions present experiments were carried out consecutively for two years (2015 and 2016) at Agricultural Research Station, Pakhribas. The experiment comprised of 11 different genotypes of garden pea including a check variety Arkel. The production performance was evaluated in a completely randomized block design with three replications. The seeds were sown at 50 × 10 cm spacing during first week of October for two years. The result showed that DGP-05 genotype had earliest 104 days after sowing. The DGP-08 genotype showed 13 which were the maximum numbers of pods per plant (13), while DGP-01 showed 8 numbers of seeds as the maximum per pod. The DGP-03 genotype had the longest pod of 9.78 cm among others. The highest fresh pod yield of 18.14 t/ha was achieved from genotype DGP-09 followed by Arkel with (16.32 t/ha).
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