Honey has been used in wound dressing for thousands of years, but only in more recent times has a scientific explanation become available for its effectiveness. The principle aim and objectives of this current work on honey was to confirm this assertion on the usefulness and medicinal value of honey. It also probed the broad-spectrum of antibacterial activity of Honey however we observed that there was much variation in potencies between different honeys from diverse sources. In this context, Gelam, Tualang and Manuka honey is used on wound complication to assess their healing potentials in which each of the honeys are applied on dressing before put on the affected lesions on the skin. Statistical analysis such as chi- square (χ2) test is used to compare association of diverse honey varieties which shows that the differences between honey varieties are not significant (P<0.05). Glucose oxidase is induced but regain its activity if the honey is diluted. It can be concluded from in vitro studies that honey has powerful antimicrobial and anti-inflammatory activities against dermatologically relevant microbes.
611 samples were collected and tested for AFB using the Zeihl-Neelsen technique. Of the 611 samples tested, 525 were negative with Ziehl-Neelsen AFB smear microscopy. The 525 negative samples were tested using the genexpert technique. 53 out of the 525 samples were positive with genexpert with a percentage positivity rate of 10.1%. The data analysis was done using the student’s t-test. There was a significant difference between the sensitivity of Ziehl-neelsen technique when compared with genexpert technique (t= 3.32; p< 0.05). Hence, there was a demonstrated increase in laboratory based TB detection using Xpert MTB/Rif compared to smear microscopy (ziehl-neelsen).
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