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In view of the increasing interest in the Methicillin-resistant Staphylococcus aureus (MRSA). The extracted DNA yield was observed using the phenol-chloroform method, it ranged from (1.6-1.8) and concentration ranged from 100 to 800 ng/µl. Five classical enterotoxin genes were investigated in 20 isolates using multiplex PCR method after it had been molecularly identified into methicillin resistant using mec A (which is the key genetic component of methicillin resistance) and fem A genes in a duplex PCR technique. A multiplex PCR test based on the simultaneous amplification of the five genes genes; sea 102bp, seb 164bp, sec 451bp, sed 278 bp and see 209bp was conducted to directly detect the toxin gene content. Our results had showed that most of MRSA samples harbored at least one enterotoxin gene. Multiple toxin gene combinations were also observed. Using this PCR assay we found that among the MRSA strains obtained (n=20).  The most commonly found gene was the enterotoxin A sea (n: 18, 90%), which was found alone and together with other toxin genes.

This research approaches to investigate toxin gene content of the methicillin resistant Staphylococcus aureus strains that are clinically collected from human skin infection cases by performing multiplex polymerase chain reaction (PCR) technique (irrespective of whether the strain produces the toxin or not) and for that a single PCR reaction was employed, two pairs of exfoliative toxin and one pair toxic shock syndrome toxin-1. Amplicon sizes ranged between 93, 226 and 326bp, to facilitate electrophoretic separation. Results of the current study had revealed that the major gene detected was tst found in six isolates. Multiple toxin gene combinations were also observed. This work was conducted at several institutions including Tikrit Teaching Hospital’s lab; college of science biology department labs/ Tikrit University; and the Biotechnology Research Centre/ Al-Nahrain University.

This study included isolating and diagnosing Staphylococcus aureus in those who diagnosed with urinary tract Infection. In this study 200, sample were collected: (100 males and 100 females). The Isolates were diagnosed based on phenotypic and microscopic characteristics and biochemical tests. In addition, the confirmatory test was conducted using API 20 staph system and 38 Isolates were obtained (23 from females and 15 from males). Some virulence factors of S.aureus Isolates were studied. These studies showed that 76.31% of the Isolates were able to produce DNase, and all isolates were bioflim-producers. The results of the isolates production of hemolysin were that 65.78% caused a full Hemolysis. Also, the ability of bacterial isolates to produce broad-spectrum beta-lactamase enzyme was studied and its production rate was 55.26% and the ability of isolates to adhering was 81.57%.

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