Our study was designed to screen the antibacterial potency of Princepia utilis leaf and seed extract and to measure their antioxidant effects, total phenol content, total flavonoid content, and total carbohydrate content. Collected samples were extracted by cold maceration. Hexane, ethyl acetate, methanol, and distilled water were used as extraction solvents. In the disc diffusion method, P. utilis ethyl acetate leaf extract was most prominent against Staphylococcus epidermis with a zone of inhibition (ZOI) of 13.83 mm. Similarly, methanolic leaf extract was most prominent against Staphylococcus aureus (ZOI-12.33 mm). Furthermore, the methanolic seed extract was most sensitive against Klebsiella pneumoniaee (ZOI-11.66 mm) Escherichia coli (ZOI-9.0 mm). The lowest minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of 0.5 mg/mL and 0.6 mg/mL, respectively, were shown by ethyl acetate leaf extract against S. epidermis. Similarly, the highest values of MIC and MBC, i.e., 20.8 mg/mL and 33.3 mg/mL, respectively, were shown by hexane leaf extract against S. epidermidis. On the other hand, evaluation of antioxidant capacity revealed that ethyl acetate leaf extract showed the maximum antioxidant effect (IC50: 66.69 μg/mL). The total flavonoid contents of different extracts were measured in the range of 37 ± 0.74 μg QE/mg dry extract weight (methanolic seed extract) to 321.84 ± 4.82 μg QE/mg dry extract weight (hexane seed extract). Likewise, the total polyphenol content ranged from the hexane leaf extract (17.33 ± 0.642 μg GAE/mg dry extract weight) to ethyl acetate leaf extract (62.56 ± 1.284 μg GAE/mg dry extract weight). We found a variation in total carbohydrate content in the range of 23.55 ± 1.125 μg glucose/mg dry extract weight (hexane leaf extract) to 96.63 ± 2.253 μg glucose/mg dry extract weight (aqueous leaf extract). Overall, this study revealed that leaf and seed extract of P. utilis exhibited noteworthy antibacterial effects against diverse pathogenic microorganisms.

This study aims to evaluate the standard physicochemical parameters, such as iodine value, acid value, saponification value, ester value, refractive index, peroxide value, and viscosity, of Prinsepia utilis (P. utilis) seed oil (PUSO) obtained as hexane extract from seeds, and to formulate ketoconazole soap (2% w/w) by using extracted oil as a base. The quality control standards of the final ketoconazole soap complied with the standards specified in Indian Pharmacopeia. Pharmaceutical soap was obtained by treating PUSO with potassium hydroxide (KOH), undergoing basic saponification. All physicochemical parameters, such as acid value (21.78 mg KOH/g), saponi-fication value (194.13 mg KOH/g), iodine value (99.7 g I2/100 g), ester value (172.35 mg KOH/g), refractive index (1.464), and viscosity (192 centipoises [cps]), conformed to industrial standards, except the peroxide value (19.23 milliequivalent KOH/g). Besides, evaluation of quality control parameters of pharmaceutical soap suggested that its various parameters, such as pH (7.3), foam-forming ability (14.5 cm), foam retention time (15 min), total fatty matter (69.31%), moisture content (10.35%), and drug content (99.37%), were within the acceptable limit. Overall, our study showed that P. utilis base was physicochemically stable and suitable for manufacturing cosmetic products, soaps, and shampoo in an economical manner, rather than using expensive chemical additives, in the pharmaceutical and cosmeceutical industry. Further, this study suggested that therapeutically and commercially successful ketoconazole soap, with all the required quality control parameters, could be manufactured by using naturally available oil at a low cost.