Introduction and Objectives: One of the vital nutrients, vitamin D, has a role in fat metabolism and other metabolic processes in addition to its direct impact on calcium and bone metabolism. This study intends to look at how vitamin D intake affects the levels of lipids, glucose, and insulin in rats with experimentally induced diabetes mellitus. Materials and Methods: 24 male albino Wistar rats, weighing between 250 to 300 g, were placed into four groups at random ( n=6). Group 1 served as the control group, while the other three groups were given an i.p. injection of 35 mg/kg streptozotocin (STZ) to induce diabetes and a high-fat diet for three weeks. Then, for the following three weeks, groups III and IV received treatment with vitamin D (400 IU/kg) and Vitamin D + Glimepiride (50 mcg/kg). Results: Vitamin D supplementation was observed to dramatically lower lipid profile, raise insulin, and decrease HbA1c concentration. Additionally, when Vitamin D and Glimepiride were administered together, a substantial decrease in the serum concentrations of blood glucose, HbA1c, and lipid profile was seen (p 0.05).