To alleviate the deleterious effect of heat stress during summer conditions on male rabbits' reproduction, 40 V Line adult rabbit bucks (on av. 8 mo old) were divided into 4 experimental groups and exposed to temperatures ranging from 23 to 36°C. Bucks in the 1 st , 2 nd , 3 rd , and 4 th group were supplemented with 0, 50, 100 or 150 mg of Chinese royal jelly (RJ)/kg twice per week, respectively, over a 20-wk period. Semen quality and blood biochemical constituents were evaluated. RJ at any dose exhibited a significant increase (P<0.05) in rabbits' sperm concentration, total sperm output, sperm motility, live sperm and normal sperm compared to the untreated controls. Plasma total protein, albumin, globulin, glucose and high density lipids (HDL) concentrations were significantly (P<0.05) boosted in the RJ groups compared to the controls. In contrast, RJ treatment resulted in a significant (P<0.05) reduction in plasma total lipids, triglycerides, cholesterol and low density lipids (LDL) concentrations. Treatment with RJ significantly boosted (P<0.05) testosterone concentration in the RJ groups to reach 110, 120 and 128%, respectively, of the control group. Improved kidney and liver functions were observed in the RJ bucks groups where plasma creatinine, urea concentrations, aspartate aminotransferase and alanine aminotransferase enzyme activities were significantly (P<0.05) decreased by RJ treatments. Treating bucks subjected to heat stress by different RJ doses increased (P<0.05) total antioxidant capacity to 106, 111 and 115% of basal, but significantly reduced (P<0.05) malondialdehyde and thiobarbituric acid reactive substances compared to the untreated. It was concluded that Chinese royal jelly supplementation for heat-stressed male rabbits can counteract summer infertility and improve their physiological status.
The aim of the present study was to investigate the long-term effect of using bee venom (BV) on the reproductive performance, immune, and health status of rabbit does and its effect on their litters. Sixty mature does, from Spanish V-line rabbit stock, were randomly assigned to four homogeneous groups with 15 does each. The 1 st , 2 nd and 3 rd groups were injected twice weekly under the neck skin with 0.1 ml solution contains 0.1, 0.2 and 0.3 mg BV/rabbit respectively. The 4 th group served as a control group. From the results, litter size at birth, litter weight and survival rate at weaning age as well as milk yield were significantly (p ≤ 0.05) increased in BV groups than in the control group. Serum estradiol 17β (E2) was significantly (p ≤ 0.05) higher (15%) in the rabbit does treated with BV compared to the control group. The treated does with BV at any study doses showed a gradual and significant (p ≤ 0.05) decrease (12%) in serum progesterone levels (P4) compared to the control. They also showed a significant (p≤0.05) increase in conception (17%) and fertility rates (10%) compared to the control does. Treatment of rabbit does with BV caused a gradual and significant (p ≤ 0.05) reduction in both aspartate aminotransferase (AST) (16%) and alanine aminotransferase (ALT) (37%) liver enzyme activities. Additionally, results have shown that BV resulted in a gradual and significant (p ≤ 0.05) increase in total antioxidant capacity (TAC), antioxidative enzymes such as glutathione S-transferase (GST) and glutathione peroxidase (GPx), serum IgG, IgM and IgA levels with significant (p ≤ 0.05) decrease in malondialdehyde (MDA) and thiobarbituric acid reactive substances (TBARS) in BV groups compared to the control group. Results suggest that BV can be used in rabbit farming as an effective and safe alternative to artificial chemical drugs (sexualstimulants) to improve certain reproductive traits, immune response and health.
Sixty low-conception rate does aged 18 to 24 months (in four groups) were used to determine the effect of replacement of prostaglandin (PG) F2α (PGF2α) injection by oral administration with sunflower oil (Sun)
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