Background: Erectile dysfunction (ED) affects a growing number of men in the USA and abroad. Phosphodiesterase 5 inhibitors as sildenafil citrate (SC) are used to treat erectile dysfunction and pulmonary hypertension. SC is mainly metabolized in the liver. Vitamin C is an antioxidant that has hepatoprotective effects. Aim of the study: To reveal the histological alterations in liver of adult and senile rats treated with SC, and to explore the impact of vitamin C co-treatment. Materials and Methods: Study was done at the House of Animals, Pharmacy Faculty, Al-Azhar University between July, and September 2021. Sixty male albino rats were used (divided in equal pattern into 5 groups); Each one was subdivided to two equal subgroups: adult and senile rats. They received drugs through oro-gastric feeding tube. Group I: received water 10 ml/ kg/ d for 30 days; group II: received 10 mg /kg /d of SC for 30 days; group III: received 26mg /d of vitamin C one hour prior to 10 mg /kg /d of SC for 30 days; group IV: received 26mg/d of vitamin C for 30 days; group V: received 10 mg /kg /d of SC for 30 days, then left one month. Then, the animals were anesthetized, and tissues of liver were proceeded and microscopically examined. Results: Histological changes were more severe in senile rats and significantly improved with vitamin C co-treatment and minimally improved in withdrawal group. Conclusion:This study revealed that vitamin C might have a protective effect against sildenafil citrate induced hepatic injury.