Interest in DNA analysis using short tandem repeats (STR) as finger printing tools in forensic medicine has gained tremendous application, as expression of these nuclear factors have enhanced forensic examination. Here we used this Biochemical characterization after conventional extraction process, polymerase chain reaction (PCR), gel electrophoresiss and a sequencer to distinguish and resolve parental dispute. The differential migration of labeled DNA fragments which attains excitation energy with a laser elicits fluorescent light of different wavelength depending on the dye used. A data collection software (Genemapper) collects raw data (spectrograph) and converts it to an electropherogram that is interpreted. By comparing the DNA profiles, inclusion and exclusion criteria were elucidated to resolve disputes. The inherent discriminating power of STRs used in analysis enhances resolution of cell mixtures, genetic aberration, substantiation of tissue origin and provides genetic distinction which is a robust and reliable approach in resolving parental disputes.

Antimicrobial resistance continues to pose serious public health challenges. OqxA and OqxB are multidrug resistance genes that confer Escherichia coli and Klebsiella pneumoniae resistance to more than one antibiotics. The purpose of this study was to detect OqxA in Escherichia coli and OqxB in Klebsiella pneumoniae from clinical samples isolated from Niger Delta University, Yenagoa. A total of 50 samples were collected. The bacterial isolates were identified using a standard bacteriological technique, the genes were detected using Polymerase Chain Reaction while the antibiotic susceptibility testing was done by disc diffusion. Of the 50 clinical isolates, 9(18%) were positive for E. coli while 15(30%) were positive for Klebsiella pnuemoniae. The total number of isolates were 18(36%) from male and 32(64%) from females. The susceptibility pattern of the isolates revealed that Escherichia coli exhibited the highest resistance of 100% to Cefuroxime and Augmentin, followed by Gentamycin and Ofloxacin (.55%) and Ciprofloxacin (11.11%) while Klebsiella pneumoniae Nalidixic Acid, Augmentin Cephalexin and Sulfamethoxazole shows 100% resistance respectively. Of the 9 that were E.coli isolates, 8(88.9%) harboured OqX A while of the 15 that were Klebsiella pneumoniae isolates, 13 (86.7%) harboured the OqxB genes. There was 100% resistance to Nalidixic acid, cefuroxime and sulfamethoxazole, with lowest resitance to meropenem (46.7%) and ciprofloxacin (60%). The report clearly demonstrates an urgent need for surveillance against these bacteria especially as they are pathogens of public health concerns to minimize the increasing pace of multidrug resistance conferred on these bacteria by OxAB genes.