During the Covid-19 pandemic, the medical team, including nurses, have struggled in isolation rooms, helping patients. Isolation rooms pose a greater risk than regular treatment rooms and direct contact can increase the risk of infection. Nurses’ anxiety may be high; they may be worried not only about the risk of transmitting Covid-19 to themselves, but also to their families at home, especially for those who are in vulnerable groups. This study aimed to identify the anxiety level of nurses in the Covid-19 isolation room at Universitas Muhammadiyah Malang Hospital. This was a descriptive observational study conducted from May 25 - June 3, 2021 with 16 nurses. The nurses’ anxiety was measured using the Hamilton Anxiety Rating Scale Questionnaire. The results showed that 13 nurses (81.25%) in the Covid-19 isolation room had mild anxiety levels. Keywords: anxiety, nurse, Covid-19, isolation room