The effects of oxidative stress on collagen and DNA biosynthesis, beta-galactosidase activity, the expression of the beta-integrin receptor, FAK, the insulin-like growth factor-I receptor (IGF-IR), the hypoxia-inducible factor-1 (HIF-1), and the mitogen-activated protein kinases (MAP/ERK(1), ERK(2)) were evaluated in human endometrial carcinoma cells. Subconfluent cells were subjected to oxidative stress with 30 microM t-butylhydroperoxide (t-BHP) for 1 h per day over the course of 5 days. It was found that oxidative stress contributed to an increase in the beta-galactosidase activity as well as to the inhibition of collagen and DNA biosynthesis. The mechanism of the process was found at the level of IGF-IR and HIF-1 alpha. An increase in the expression of HIF-1 alpha and a decrease in the expression of IGF-IR were observed in the cells subjected to oxidative stress. The role of IGF-IR signalling in the process was confirmed by an experiment showing downregulation of MAP kinases ERK(1) and ERK(2) expression in the studied cells. This phenomenon is probably responsible for the drastic inhibition of protein (up to 40 % of control) and DNA biosynthesis (up to 65 % of control) in the cells. An addition of tiliroside to the cells medium restored all parameters to the control level, including IGF-IR and HIF-1 alpha expressions. The data suggest that the antioxidative activity of tiliroside isolated from Potentilla argentea may originate at the level of IGF-IR and HIF-1 alpha signalling.

To enhance the safety of the transport of dangerous goods in tanks, the latter should improve the mechanical (physical) protection to prevent accidental damage to the boilers, such as objects containing dangerous goods directly. Damage to the boiler will almost inevitably leads to such consequences as decompression, the leakage of liquid radioactive waste and radiation pollution of the surrounding area. This article discusses scenarios accidents when overloading with marine railway transport; the explosion of gas cylinders on board the vessel. Calculation formulas are obtained to determine the speed at which the shards is provided by probabilistic the breakout a certain thickness of the tank walls. Received probability distribution function for the specified parameters shards and material barriers, which was determined by conducting a full-scale experiment. The results of theoretical studies, experiments used to improve designs of tanks carrying LRW and recommendations for improving the security of multimodal transport.

The article identifies the main problems of eliminating the reduction of environmental damage by improving the response systems to man-made accidents at hazardous production facilities of the oil and gas complex. Risk factors for ensuring safety at a hazardous production facility in the oil and gas industry are identified, the most significant risk factor is identified by the method of hierarchical analysis, and the problem of the engineering and technical factor in the system “man technical environment industrial environment environment” is revealed. “The solution to the problem is indicated the use of a developed mobile emergency response system, which makes it possible to halve the response time to an emergency. The developed response system can be applied at any hazardous production facility in the oil and gas industry due to its flexibility.

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The article is devoted to the identification and analysis of the physical characteristics and thermal effects of a fire in an emergency situation. The main purpose of the work is to study the influence of physical damaging factors on the degree of danger of a potentially hazardous object. The article considers a deterministic method for assessing the effects of the action of a physical damaging factor (intensity of thermal radiation). The study is an effort to analyze the physical damaging factors of the fire, because it is the physical basis for an objective assessment of the danger and risk of an accident at the researched object.