Highly purified liver nuclei incorporated radiolabeled phosphate into phosphatidylinositol 4-phosphate (PtdIns(4)P), PtdIns(4,5)P 2 , and PtdIns(3,4,5)P 3 . When nuclei were depleted of their membrane, no radiolabeling of PtdIns(3,4,5)P 3 could be detected showing that within the intranuclear region there are no class I phosphoinositide 3-kinases (PI3K)s. In membrane-depleted nuclei harvested 20 h after partial hepatectomy, the incorporation of radiolabel into PtdIns(3)P was observed together with an increase in immunoprecipitable PI3K-C2␤ activity, which is sensitive to wortmannin (10 nM) and shows strong preference for PtdIns over PtdIns(4)P as a substrate. On Western blots PI3K-C2␤ revealed a single immunoreactive band of 180 kDa, whereas 20 h after partial hepatectomy gel shift of 18 kDa was noticed, suggesting that observed activation of enzyme is achieved by proteolysis. When intact membrane-depleted nuclei were subjected to short term (20 min) exposure to -calpain, similar gel shift together with an increase in PI3K-C2␤ activity was observed, when compared with the nuclei harvested 20 h after partial hepatectomy. Moreover, the above-mentioned gel shift and increase in PI3K-C2␤ activity could be prevented by the calpain inhibitor calpeptin. The data presented in this report show that, in the membrane-depleted nuclei during the compensatory liver growth, there is an increase in PtdIns(3)P formation as a result of PI3K-C2␤ activation, which may be a calpain-mediated event.

Introduction: The aim of this research is centered on phytochemical analysis and anti-inflammatory activity of Cladophora aegagropila extract. Methods: Total flavonoid content in the appropriate ethanol extract of C. aegagropila was determined using the spectrometric method. Anti-inflammatory activity was evaluated by the models of carrageenan-induced and allyl isothiocyanate-induced (AITC-induced) inflammation of hind limb in rats. Experimental animals were divided into 3 groups, 5 animals each: 1st group – control; the animals without therapy of induced inflammation, 2nd group – animals were treated with application of 5% ointment containing C. aegagropila; 3rd group – animals were treated with application of 5% ibuprofen. Evaluation of anti-inflammatory activity was performed by determination of dynamic change of thickness and volume of affected animal limb for carrageenan-induced inflammation for 8 days (with daily registration of records), and for AITC induced inflammation for 24 h (with registration of records after 30 min, 1 h, 2 h, 3 h, 6 h and 24 h since phlogogen was introduced). Results: Flavonoids content in C. aegagropila extract varied depending on ethanol concentration: usage of 40% ethanol led to 18.2 mg of flavonoid recovery on 1.0 g of dry raw material; 70% ethanol – 39.5 mg and 96% ethanol – 35.5 mg. Therapy of 5% ointment based on C. aegagropila extract decreased inflammatory response caused by the subplantar introduction of the corresponding phlogogen. Conclusion: The ointment from C. aegagropila extract shows anti-inflammatory activity by inhibiting inflammation caused by AITC and carrageenan.

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In media text on international relations, disagreement between countries is presented metaphorically as a disagreement between people.The relation between metaphor and discourse is studied by Zinken and Musollf (2009). Mussolf studies metaphors related to the EU organized in “scenarios”. In his view, the thematic target (for instance, EU politics) is accessed through a source input for the metaphor complex (family/marriage/concepts) (Mussolf 2006) and this is “characterized by the dominance of a few traditional, gender-coded stereotypes of family roles” (Mussolf 2009: 1).The present paper traces the ways disagreement in the sphere of international relations is presented in the media.In this study, the observed patterns used to represent disagreement between countries are argument, disagreement, conflict, and fight. The level of disagreement varies depending on the metaphoric scenario used to represent it. It was observed that the strongest way of expressing disagreement is based on the “split up”, and “break up” scenario, followed by the “fight”, “conflict” and the “argument” scenario.In expressing disagreement in media text on international affairs, Lakoff’s STATE IS A PERSON metaphor (Lakoff 1990, 1995) is used. In Chilton and Lakoff’s view, metaphors are not mere words or fanciful notions, but one of our primary means of conceptualizing the world. As they have stated, a metaphor is “a means of understanding one domain of one’s experience in terms of another” (Chilton, Lakoff 1989). Member states are presented as people who quarrel and disagree over issues related to international relations or policies. Along with that metaphor, a place for the institution metonymy is used. As Barcelona has stated, proper names are often metonymic in origin, i. e. they refer to a circumstance or distinctive aspect linked to their referent (Barcelona 2004, 2005).The place for the institution metonymy is found in two variants: the country for the institution and the capital for the institution. For instance, a disagreement between the governments of two countries is presented as disagreement between their capitals, as in “Paris and Berlin fundamentally “disagree” on who should succeed Jean-Claude Juncker” (https://www.express.co.uk)”. The same situation is presented as a disagreement between countries: „Germany and France ‘DISAGREE’ over Juncker replacement” (ibid). In the abovementioned examples, an item from one of the two metonymic chains is juxtaposed to a corresponding item in the other chain:Paris (place name - capital) — Berlin (place name - capital)Germany (place name- country) — France (place name- country)It seems that names from one metonymic chain belonging to a certain class of names (country name, names of cities, capitals, regions, continents, etc.) are juxtaposed to names from another metonymic chain, belonging to the same class of names. However, there are texts in which this is not necessarily the case. A name of city (capital) is often juxtaposed to a name of a country, as in “Paris put its foot down, and won’t let Germany get its way” (www.politico.eu). Expressions may vary depending on the stregth of disagreement, ranging from “disagree”, “argue”, “conflict” to “fight’, “split up” and “break up”.

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