No abstract

Environmental pollution resulting from oil exploitation and exploration has impacted negatively on the biodiversity of the affected areas. Therefore, this study investigated the response of soil microorganisms to oilfield wastewater. The oilfield wastewater and soil samples were collected from an onshore oil producing platform fortnightly for a period of three months and microbiological analyses were performed using standard methods. Soil around pond average counts for THB and HUB (Log10 CFU/g) were 7.32 to 7.35 and 4.16 to 4.22 respectively. Soil 80 m away from pond average range for THB and HUB were 7.38 to 7.40 and 3.32 to 3.34 respectively. For TF and HUF, Soil around the pond mean recorded 4.65 to 4.85 and 4.12 to 4.16 (Log10 CFU/g) respectively. Mean monthly counts for soil 80m away TF and HUF (Log10 CFU/g) were 5.03 to 5.05 and 3.26 to 3.34 respectively. Soil around the pond recorded lower total heterotrophic bacteria count and total fungi count but higher hydrocarbon utilizing bacteria and hydrocarbon utilizing fungi than soil 80 m away. There was significant difference (P<0.05) between the THB and HUB in the various samples. For the fungi count there was no significant difference (P>0.05) between TF and HUF in all the samples except in sample obtained from soil 80 m away from pond. Bacteria species isolated from the study include: Bacillus, Aeromonas, Micrococcus, Staphylococcus, Chryseomonas, Proteus, Pseudomonas, Klebsiella. Apart from Aeromonas and Chryseomonas the rest of the isolate were identified also as hydrocarbon utilizing bacteria. While fungi species isolated includes: Aspergillus fumigates, Aspergillus niger, Aspergillus flavus, Saccharomyces cerevisiae, Geotricum, Trichoderma, Fusarium and Penicillium. Hydrocarbon utilizing fungi that occurred includes: Aspergillus niger, Aspergillus flavus, Fusarium, Penicillium and Saccharomyces cerevisiae. The response of these microorganisms in the oil polluted environment suggests that the isolated bacteria and fungi could utilize the oil as energy and carbon source and could be effective in the cleanup of the polluted sites.

No abstract

Foodborne contamination especially by Staphylococcus species is of concern as many isolates of the genus have been reported for their multidrugs resistance. The study was aimed at determining the antimicrobial resistance of Staphylococcus species isolated from abattoir. A total of 100 samples were collected from five different surfaces; bucket swab, butchers hand swab, table swab, knife swab and meat swab from four abattoirs in of Rivers State, Nigeria. The samples were transported aseptically to laboratory for immediate analysis. The samples were cultured on mannitol agar (MSA) using standard microbiology technique to isolate Staphylococcus species. Hemolysis test was carried out and susceptibilities of isolates against a panel of 10 antibiotics were determined using the Kirby-Bauer disk diffusion method, and the multiple antibiotic resistance (MAR) index of the isolates were determined. Out of the 43 isolates of Staphylococcus from the abattoirs, 51.1% were coagulase positive while 48.9% were coagulase negative. 18.6% of the Staphylococcus species produced beta hemolysis and 16.28% showed alpha hemolytic activity. Staphylococcus species produced resistance to amoxicillin (88.4%), zinnacef (88.4%), ampiclox (88.4%), receptrin (60.5%), streptomycin (37.2%), septrin (37.2%), erythromycin (34.5%), and gentamicin (39.5%), ciprofloxacin (20.9%) and perfloxacin (16.8%). The multidrug resistance index (MAR index) showed that 2.35% of the isolates showed complete resistance to all the 10 antibiotics tested with MAR index of 1, 4.6% recorded MAR index of 0.1, 4.6% recorded MAR index of 0.2. Majority of the isolates of Staphylococcus species associated with meat and materials or equipment (such as bucket, knife) used in abattoir and the hands of butcher are multidrug resistance and is of great medical concern hence there is need to regulate the indiscriminate use of antibiotics.

No abstract