The development of insects is strongly influenced by their resident microorganisms. Symbionts play key roles in insect nutrition, reproduction, and defense. Bacteria are important partners due to the wide diversity of their biochemical pathways that aid in the host development. We present evidence that the foam produced by nymphs of the spittlebug Mahanarva fimbriolata harbors a diversity of bacteria, including some that were previously reported as defensive symbionts of insects. Analysis of the microbiomes in the nymph gut and the soil close to the foam showed that the microorganisms in the foam were more closely related to those in the gut than in the soil, suggesting that the bacteria are actively introduced into the foam by the insect. Proteobacteria, Actinobacteria, and Acidobacteria were the predominant groups found in the foam. Since members of Actinobacteria have been found to protect different species of insects by producing secondary metabolites with antibiotic properties, we speculate that the froth produced by M. fimbriolata may aid in defending the nymphs against entomopathogenic microorganisms.

No abstract

Significance and Impact of the Study: Inspired by the environmental concern associated with the use of fossil fuels, several countries are encouraging the development of biofuels. Sugarcane cultivation supports Brazil as one of the largest world sugar and ethanol producer. In the last decades, we observed a change on soil-plant system in the productive system (manual to mechanized), but the impact of this change is underestimated. Soil quality was evaluated using microorganisms associated with the nitrogen cycle, and it was possible to observe that these biological tools were more sensitive proxies to indicate soil responses to different management strategies.