Six peach and six nectarine cultivars were evaluated for the phenolic content in their pulp and peel tissues. Chlorogenic acid, catechin, epicatechin, rutin and cyanidin‐3‐glucoside were detected as the main phenolic compounds of ripened fruits. The concentration was always higher in peel tissue, with average values ranging from 1 to 8 mg g−1 dry weight (DW) depending on cultivar. Of the tested varieties, the white‐flesh nectarine ‘Silver Rome’ emerged as the cultivar with the highest amount of total phenolics. Phenolic compounds were also profiled during fruit growth and ripening in the yellow nectarine cv. ‘Stark Red Gold’, which showed a decreasing concentration during fruit development in both peel and pulp tissues. Average amounts of total phenolics were approximately 25 mg g−1 DW 60 days after full bloom and decreased to 3 mg g−1 DW at ripening in pulp tissue. Differences among peel and pulp composition show the different dietetic and antioxidant potential of fruits consumed unpeeled and peeled.

A chiral CD-MEKC method, enantioselective for catechin and gallocatechin, was developed, validated and applied to the analysis of tea samples. The method was addressed to the fast and simultaneous quantitation of the most represented and biologically important green tea catechins and methylxanthines. The CD-MEKC was based on SDS as surfactant (90 mM) and hydroxypropyl-beta-CD (25 mM) as chiral selector, under acidic conditions (25 mM borate-phosphate buffer, pH 2.5). The method was first applied to study the thermal epimerisation of epi-structured catechins, (-)-epicatechin and (-)-epigallocatechin, to non-epi-structured (-)-catechin and (-)-gallocatechin. The latter compounds, being non-native molecules, were for the first time regarded as useful phytomarkers of tea samples subjected to thermal treatment. The proposed method was applied to the analysis of more than twenty tea samples of different geographical origins (China, Japan, Ceylon), having undergone different storage conditions and manufacturing processes. Finally, factor analysis was used to visualise the useful information contained in the data set, showing that it was possible to distinguish tea samples on the basis of their different contents of native and non-native catechins.

This paper explores the possibility of using biodegradable cross-linked gelatines as antibiotic devices for a long-term elution (80 days). Capillary electrophoresis (CE) has been utilized to evaluate the mass percentage of vancomycin and gelatine contemporary released from differently cross-linked vancomycin loaded gelatine samples in an elution time ranging from 24 to 1920 h. While the solubilization kinetic of gelatine samples differently cross-linked can be very close described by the simplified Higuchi model, the vancomycin release kinetic is contemporary governed by both the Fickian diffusion process trough the gelatine matrix network and the dissolution process of the matrix due to its degradation. Comparing the antibiotic eluting kinetics from gelatine at diverse cross-linking degree we observed that the degradation of the proteic matrix appears to have a minor influence in the drug release control. Vancomycin released from all the gelatine partially cross-linked samples results active against Staphylococcus aureus and Streptococcus faecalis which represent the most pathogens commonly isolated in orthopaedic infections. Vancomycin overcomes the minimum inhibitory concentration for both the bacteria in the whole range of elution time. Cross-linked gelatine devices appear to represent a useful biodegradable delivery system for local anti-infective therapy in arthoplasty.

The efficacy of the dioxygenase inhibitor prohexadione‐Ca (ProCa) as an inducer of resistance responses against apple scab (Venturia inaequalis/Spilocaea pomi) was evaluated in two sets of experiments under glasshouse conditions. ProCa (200 ppm) was applied on seedlings of open‐pollinated Malus domestica L. cv. Golden Delicious, and 10 days after application, a group of seedlings was inoculated with a conidial suspension of S. pomi. Total RNA was extracted from leaf samples collected at different times from both inoculated and noninoculated seedlings, and expression of the pathogenesis‐related proteins PR‐1, PR‐2, PR‐5, PR‐8, PR‐10 and chitinase was studied by reverse transcription–polymerase chain reaction and Northern blot hybridisation. Disease severity was significantly lower on seedlings treated with ProCa than on the water‐treated control seedlings. ProCa triggered a general increase in gene expression of almost all the PR genes; only the level of chitinase and PR‐10 did not show a detectable increase. A strong upregulation of all the assayed genes was observed in leaves of apple seedlings first treated with ProCa and then inoculated with the pathogen, whereas no expression and/or a constitutive signal was found in samples from the control seedlings.

No abstract