Methicillin resistance is relatively common in Staphylococcus isolates from dogs with corneal infections, particularly among dogs belonging to healthcare workers.
Objective
To assess the reproducibility and repeatability of an optical coherence tomography (OCT) device for imaging the optic nerve region of normal canines.
Animals studied
Twelve clinically healthy Beagles.
Procedures
All animals were anesthetized and an OCT device was used to image the optic disc region. Total disc area and retinal nerve fiber layer (NFL) thickness in eight segments were obtained from each image. Images were collected by two operators in succession and each operator took five scans, repositioning the device between measurements. B-scan segmentations and disc outlines were subsequently redrawn to obtain manual measurements, allowing for comparisons between automated measurements.
Results
Operator 1 had a mean (±SD) NFL thickness (automatic) and disc area of 73.38 ± 7.41 µm and 3.69 ± 0.52 mm2, while operator 2 had 74.27 ± 7.33 µm and 3.67 ± 0.51 mm2, respectively. With manual corrections, operator 1 had a NFL thickness and disc area of 86.19 ± 8.26 µm and 3.74 ± 0.68 mm2 while operator 2 had 86.85 ± 6.91 µm and 3.81 ± 0.56 mm2, respectively. Operators did not obtain significantly different values for any region. Intra-class correlation ranged from 0.33–0.97 (average 0.78). Coefficient of variation ranged from 8.8–36.2%.
Conclusion
The OCT device demonstrated high inter-rater reliability. Intra-rater reliability was strong for disc area and total NFL; however the segmental regions demonstrated a higher degree of variability. Manual and automated measurements were significantly different for total NFL and some segmental regions therefore users should consistently use one method for longitudinal studies.
OBJECTIVE To assess and compare 2 injection techniques for conducting ocular anterior segment indocyanine green angiography (ASICGA) and sodium fluorescein (SF) angiography in horses. ANIMALS 3 healthy adult female horses (age range, 19 to 25 years). PROCEDURES Horses were sedated, jugular catheters were placed, and manual restraint was used to ensure proper positioning for the angiography procedure. Two injection techniques (IV and intra-arterial) were performed for each horse 1 week apart. Intravenous injections of 0.25% indocyanine green (ICG; 50 mg) and 10% SF (10 mg/kg) were administered via the jugular catheter. Intra-arterial injections of ICG (1 mg) and SF (1 mg/kg) were administered into the common carotid artery with ultrasound guidance. Angiography was performed by use of an adaptor system comprised of a modified digital single-lens reflex camera, camera adaptor, and lens. Imaging was performed at a rate of 3 images/s for 60 seconds immediately following ICG injection, then at 2, 3, 4, and 5 minutes after injection. The SF was injected 5 minutes thereafter. RESULTS ASICGA allowed visual identification of the arterial, capillary, and venous phases of angiography. Intra-arterial administration provided superior dye fluorescence, sharper contrast, and faster dye passage than IV administration. Visibility of the iris vasculature was limited with SF, and extravasation of SF was noted. No clinically important adverse events were detected. CONCLUSIONS AND CLINICAL RELEVANCE ASICGA images were obtainable with both injection techniques; however, visibility of the iris vasculature was better with intra-arterial administration of ICG. The ASICGA technique may serve as a viable ocular imaging modality for horses.
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