Understanding the sources and controls on microbial methane production from wetland soils is critical to global methane emission predictions, particularly in light of changing climatic conditions. Current biogeochemical models of methanogenesis consider only acetoclastic and hydrogenotrophic sources and exclude methylotrophic methanogenesis, potentially underestimating microbial contributions to methane flux. Our multi-omic results demonstrated that methylotrophic methanogens of the family Methanomassiliicoccaceae were present and active in a freshwater wetland, with metatranscripts indicating that methanol, not methylamines, was the likely substrate under the conditions measured here. However, laboratory experiments indicated the potential for other methanogens to become enriched in response to trimethylamine, revealing the reservoir of methylotrophic methanogenesis potential residing in these soils. Collectively, our approach used coupled field and laboratory investigations to illuminate metabolisms influencing the terrestrial microbial methane cycle, thereby offering direction for increased realism in predictive process-oriented models of methane flux in wetland soils.

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Acute idiopathic maculopathy (AIM), originally termed unilateral AIM, is a rare inflammatory disorder that typically affects young adults (mean age, 32 y; range, 15 to 45 y) and is characterized by acute, severe, unilateral vision loss preceded by a flu-like illness. Men and women are affected equally. Patients generally present with visual acuity of r20/200 and complain of central metamorphopsia and/or scotoma formation that is attributable to an exudative macular detachment. There may also be hypopigmentation of the retinal pigment epithelium, which may simulate the appearance of choroidal neovascularization (CNV) (Fig. 1). 1 Papillitis, a few vitreous cells, subretinal exudation, and bilaterality have also been observed in AIM, whereas eccentric macular lesions have been noted in cases with milder vision loss (20/60 to 20/100). 2 Most cases resolve spontaneously with near-complete recovery of vision (20/25 or better) over a period of 3 weeks to 6 months. After resolution of the macular detachment, a ''bull's-eye'' pattern composed of retinal pigment epithelial (RPE) hyperpigmentation surrounded by hypopigmented atrophic changes develops at the site of the antecedent neurosensory detachment (Fig. 2). To date, there has been only 1 reported case with recurrent episodes of AIM. 3 Rarely, secondary CNV has occurred after resolution of AIM. The authors have seen a case of secondary CNV after AIM that responded well to intravitreal anti-vascular endothelial growth factor therapy.The flu-like illness that precedes AIM suggests a viral cause. The coxsackie virus has been implicated in the pathogenesis of AIM,