Watu Joglo is one of the caves located in the row of the Sewu mountains, Gunung Kidul, Special Regency of Yogyakarta. Watu Joglo has various suitable microhabitats for herpetofauna. So far, the study about herpetofauna’s community structure and distribution has not been carried out in Watu Joglo. This study aimed to study and map the diversity, richness, evenness, and distribution of herpetofauna in Watu Joglo. Herpetofauna’s data sampling was carried out for three days. The study was conducted on 6-8 April 2018. The data of herpetofauna community were analyzed with Shannon-Wiener diversity index, Margalef richness index, and Pielou Evenness Index. The data of herpetofauna distribution were mapped and analyzed with Avenza and ArcGis. Based on the research conducted, we found 11 species of herpetofauna which consisted of five species from Ordo Anura, four species from Subordo Lacertilia, and two species from Subordo Serpentes. Herpetofauna in Watu Joglo was widely distributed around the river. According to diversity analysis, Watu Joglo has moderate diversity index, 1.667. Besides, Watu Joglo has high evenness index which is 0.85 but low richness index which is 2.83.

Banyu Nibo Waterfall is located near Nglanggeran, Gunung Kidul that well known for its eco-tourism development. The geographical and ecosystem condition could be providing unique habitat for herpetofauna. Herpetofauna has important role in the ecosystem as food chain components and even as environment bioindicator.The aim of this research is to asses the herpetofauna diversity in Banyu Nibo Waterfall as preliminary biodiversity data.  The research was conducted using VES (Visual Encounter Survey) assisted by 500 meters transect line. As a result, 15 species were found, mainly distributed on area with tree coverage.