No abstract

No abstract

PROTEIN COMPONENT OF ENDOTOXINblood glucose must be maintained for a certain period before its reduction to stimulate HGH secretion.Oufr observation is difficult to interpret, but raises some questions on the regulatory manner of HGH secretion mlediated by glucose metabolism. If the stimulation of HGH secretion CUTS by intracellular glucose deprivation( 1-3) in the hypothalamus( 2,7) as proposed, glucose infused for a short period in most cases is unable to induce enough metabolic shift in the hypothalamic cells to stimulate HGH secretion, in spite of other definite responses such as plasna NEFA change. An alternative explanation may be that the direct stimulant of HGH secretion is not the extracellular or intracellular glucose itself, but the metabolites or other substances related to glucose metabolism, which are different quanrtitatively or qualitatively in the conditions of Short and long continuous glucose infusion. Since plasma NEFA change was similar in all cases, it is apparent that there is no conrelation between the rise of plasma HGH and the value of plasma NEFA. In these respects, our results may support the observation of Quabbe et aZ (8), who showed the absence of correlation between plasma HGH rise and blood glucose or plasma NEFA level during a 24-hour fast in normal subjects.Summary. Serial plasma HGH concentrations were measured, in 18 normal subjects, following continuous intravenous glucose in-fusion performed for periods of 3, 10, 20 and 30 minutes. In 5 of 8 cases infused for 3 minutes, no rise of plasma HGH was olbserved in spite of the rapid fall of blood glucose with (4 cases) or without (1 case) its reduction below the fasting level. The 3 other cases infused for 3 minutes and all 10 cases of 10, 20 and 30 minutes' infusion showed definite rise of plasma HGH at 90-180 minutes following the infusion. From the results obtained, it appears that the falling blood glucose per se is not a direct stimulus, and the reduction of blood glucose !below the fasting level is not necessarily a stimulus to HGH secretion.

A Boivin preparation of Brucella abortus, unlike common enterobacterial endotoxins, failed to depress water intake or increase numbers of hemolysin-producing spleen cells in mice, or to cause delayed inflammatory reactions in rabbit skin. Reactivity to the B. abortus endotoxin was found only in animals which were previously given the endotoxin with, but not necessarily in, complete Freund's adjuvant. Previous treatment with the endotoxin in saline or with only the adjuvant was ineffective. Sensitization appeared within 10 days and waned after 5 weeks. Passive sensitization was obtained with sensitized donor spleen cells but not with serum. Serum antibody titers did not correlate with the appearance and disappearance of sensitization.

No abstract