Reports about acquired resistance to colistin in different bacteria species are increasing, including E. coli of animal origin, but reports of resistance in wild S. enterica of different serotypes from swine are not found in the literature. Results obtained with one hundred and twenty-six E. coli strains from diseased swine and one hundred and twenty-four S. enterica strains from diseased and carrier swine showed a frequency of 6.3% and 21% of colistin-resistant strains, respectively. When comparing the disk diffusion test with the agar dilution test to evaluate the strains, it was confirmed that the disk diffusion test is not recommended to evaluate colistin resistance as described previously. The colistin MIC 90 and MIC 50 values obtained to E. coli were 0.25 μg/mL and 0.5 μg/mL, the MIC 90 and MIC 50 to S. enterica were 1 μg/mL and 8 μg/mL. Considering the importance of colistin in control of nosocomial human infections with Gram-negative multiresistant bacteria, and the large use of this drug in animal production, the colistin resistance prevalence in enterobacteriaceae of animal origin must be monitored more closely.
BackgroundInfluenza A viruses circulating in pigs in Brazil are still not characterized, and only limited data are available about swine influenza epidemiology in the country. Therefore, we characterized the hemagglutinin (HA) and neuraminidase (NA) genes of influenza viruses isolated from Brazilian pigs. We also evaluated one case of probable swine‐to‐human transmission.MethodsTwenty influenza viruses isolated from pigs during 2009–2010 in five Brazilian states (Minas Gerais, Sao Paulo, Parana, Rio Grande do Sul, and Mato Grosso) were used. One human isolate, from a technician who became ill after visiting a swineherd going through a respiratory disease outbreak, was also used in the study. Phylogenetic analysis for the HA and NA genes and hemagglutinin amino acid sequence alignment were performed.ResultsAll isolates clustered with pandemic H1N1 2009 (pH1N1) viruses and appeared to have a common ancestor. Genetic diversity was higher in the HA than in the NA gene, and the amino acid substitution S203T in one of HA's antigenic sites was found in most of the samples. The human isolate was more related to swine isolates from the same herd visited by the technician than to other human isolates, suggesting swine‐to‐human transmission.ConclusionOur results show that pH1N1 was disseminated and the predominant subtype in Brazilian pigs in 2009–2010.
A total of 110 strains of Streptococcus suis, isolated from diseased pigs in Brazil were serotyped and analyzed for virulence. Serotyping of the strains resulted in the following classification: 42 strains of serotype 2 (38.2%), 10 strains of serotype 14 (9.1%), seven strains of serotype 9 (6.4%), three strains each of serotype 7 and 11 (2.7%), two strains each of serotype 1 and 8 (1.8%) and one strain each of serotypes 1/2, 3, 5, 6 and 10 (0.9%). Cross reactions among serotypes 1, 14 and 7 were observed in 21 strains (19.1%). Only 41.9% of the strains were lethal for mice using the pathogenicity test.
The production of large quantities of paint sludge is a serious environmental problem. This work evaluates the use of pyrolysis reaction as a process for deactivating paint sludge that generates a combustible gas phase, a solvent liquid phase and an inert solid phase. These wastes were classified into three types: water-based solvent (latex resin) and solvents based on their resins (alkyd and polyurethane). An electrically heated stainless steel batch reactor with a capacity of 579 mL and a maximum pressure of 30 atm was used. Following the reactor, a flash separator, which was operated at atmospheric pressure, partially condensed and separated liquid and gas products. Pressure and temperature were monitored on-line by a control and data acquisition system, which adjusted the heating power supplied to the pyrolysis reactor. Reactions followed an experimental design with two factors (reaction time and temperature) and three levels (10, 50 and 90 minutes; 450, 550 and 650°C). The response variables were liquid and solid masses and net heat of combustion. The optimal operational range for the pyrolysis process was obtained for each response variable. A significant reduction in total mass of solid waste was obtained
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