Hypothyroidism is the most common long-term consequence after total thyroidectomy. The objective of the present study was to evaluate the lipid profile and liver function in patients after hypothyroidism. Sixty patients who underwent a surgical operation to remove thyroid were included in this study, and thirty healthy subjects were used as a control. The study was conducted at Al-Sadr Medical City in Al-Najaf city, in Iraq, from October 2020 to March 2021. Thyroid-stimulating hormone (TSH) was very high in patients at a hypothyroid stage after hypothyroidism. The results showed a significant increase in total cholesterol, triglycerides (TG), low-density lipoprotein (LDL), and the ratio of total cholesterol/high-density lipoprotein (HDL). The study also revealed a significant increase in the liver enzymes aspartate aminotransferase (AST) and alanine transaminase (ALT) and a significant decrease in alkaline phosphatase (ALP) in patients with thyroidectomy compared to the control group. The correlation matrix revealed a strong positive correlation between TSH levels and total cholesterol, triglycerides, LDL, AST, ALT, and ALP. It was concluded that hypothyroidism, the major consequence of thyroidectomy, causes dysfunction in lipid metabolism and liver enzymes resulting in secondary hyperlipidemia and liver dysfunction.

Background: The study of ASA etiology is very important in the diagnosis and treatment of infertility. Studies of presence of antisperm antibodies in the bodies of unmarried women are very rare, so that this article aims at studying the possible causes and interpretations behind the development of antisperm antibodies in virgins. Methodology: The study included 5 single women with positive ASA. Description and clinical history of the patients was assessed by special questionnaire provided for this purpose. All laboratory investigations and diagnostic procedures were done in the hospital from 1st August to 15th December 2017. Results: The mean serum antisperm antibody concentrations (64.3 IU/ml) which is considered positive titer. The incidence of ASA among virgins is 2.22%. Complete blood count is normal except for slight increase in WBC count and percentage of basophils, monocytes and lymphocytes. This study recorded high concentrations of serum total IgG and IgM levels (1875 IU/ml and 295 IU/ml respectively). The UTI was confirmed by counting total bacterial concentration (178,250 CFU/ml) in the urine, and diagnosis of suspected causes showed the following species: Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, and Proteus mirabilis. Conclusions: There are two suggested mechanisms to explain ASA in virgins: 1) antigen cross-reactivity between sperm and bacterial antigens to which antibodies can react; 2) induction of the immune system by antigens of sperm ingested into the gastrointestinal tract with contaminated food and drink. Recommendations: it is recommended to conduct a research study that include a large number of virgins for investigating ASA to confirm our results and build a scientific generalizations , in addition to animal studies for testing the role of sperm ingestion in the induction of immune system.

Vitamin C ascorbic acid is a well-known antioxidant that is involved in anxiety, stress, depression, fatigue and mood state in humans. The purpose of this study is to investigate the effect of vitamin C on decreasing the level of public speaking stress in term of heart rate, blood pressure and stress assessment. A purposive sample of (45) were included in this randomized placebo-placebo trial study, that was conducted at Faculty of nursing / university of Kufa. Public speaking stress was determined by a questionnaire scale developed from (public speaking anxiety scale). Study group included volunteer students who took a dose of 500 mg a day of vitamin C for one week before a group presentation, while the placebo group is given a placebo before one week of before a group presentation. The result indicated that the blood pressure (systolic and diastolic) before presentation between the two groups ( student group and placebo) was not significantly different. In relation to heart rate, a significant decrease (p< 0.05) was recorded in student group as compared to placebo. The student group has recorded significant difference in many items of stress assessment when comparing to placebo (e.g. increase of heart rate, dry mouth, GIT cramps and anxiety due to fear from failure). The assessment of presentation stress between the two groups (student group and placebo) was determined by means of scores. The results revealed that there is a significant difference (P< 0.05) in presentation stress between student group and placebo group. It was concluded that student group which took 500 mg vitamin C for one week express less presentation stress. The most stress signs that has been improved by vitamin C intake are: increase of heart rate, dry mouth, GIT cramps and anxiety due to fear from failure.

Background: It is a scientific fact today that routine seminal analysis is only a poor predictor of male reproductive potentiality, so that there is an increased need for finding out new functional tests in the assessment of male infertility. Objective: The aim of the present study was to investigate the predictive value of sperm chromatin integrity assays as a complementary tool to assist in diagnosis of infertility. Methodology: This study was carried out between January 2013 and November 2013 including one hundred (100)selected infertile men who attended to fertility Center in Al-Sadr Medical City. The study also included twenty (20) healthy fertile volunteers as a control. Aniline blue (AB) staining was used to differentiate between normal condensed chromatin (unstained) and abnormal decondensed chromatin (blue), while toluidine blue (TB) was use to differentiate between normal intact sperm DNA (blue) and abnormal damaged sperm DNA (purple), this is the first study in Iraq that employed (TB) to assess sperm DNA fragmentation. Results: The results showed that there was a high significant difference (p<0.01) in sperm chromatin decondensation and sperm DNA damage between infertile and fertile men. The study also revealed a significantdifference (p<0.05) in sperm DNA damage between normozoospermic and fertile men, but no significant difference (p>0.05) was found in the sperm chromatin condensation. Both sperm chromatin condensation and sperm DNA damage showed a significant positive correlation with sperm morphology, and a significant negative correlation with sperm concentration, motility and progressive motility. Conclusions: The study concluded that sperm DNA fragmentation (by TB staining) has good predictive value in discriminating infertile and fertile men over seminal fluid analysis. Recommendations: TB and AB staining of sperm chromatin can be used in the course of infertility diagnosis. However, more investigations may be needed to confirm the discriminating power of sperm chromatin condensation assays (by AB staining) to be ready to use in the routine workup in the fertility clinics.

In the case of comparison between samples with unequal replications [n 1 # n 2 # n 3 # ••• # n k ], the statistician should use LSD (Least Significant Difference) many times to achieve these comparisons, this method consumes more time and effort. The purpose of this research is to find out a new method to do easy and reliable comparison between population means when the experiment or data involving different numbers of replications. In order to do this, a new formula was designed; the aim of this new formula is to develop the multiple comparisons into one step instead of doing it with many steps that consume more effort and time. In this work, the researcher used the mean of the replication number in the LDS formula instead of doing the calculation many times for each two means. The results of this study found that the new formula give similar results for multiple comparisons with minimal effort. It was concluded that the new formula (named Al-fahham's Formula) achieves the same results with less time and efforts. It was recommended that Al-fahham's Formula can be approved in the statistical textbooks and procedures.