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Genetic programming (GP) is presented as a new tool for the estimation of reference evapotranspiration by using daily atmospheric variables obtained from the California Irrigation Management Information System (CIMIS) database. The variables employed in the model are daily solar radiation, daily mean temperature, average daily relative humidity and wind speed. The results obtained are compared to seven conventional reference evapotranspiration models including: (1) (7) the Turc method. Statistical measures such as average, standard deviation, minimum and maximum values, as well as criteria such as mean square error and determination coefficient are used to measure the performance of the model developed by employing GP. Statistics and scatter plots indicate that the new equation produces quite satisfactorily results and can be used as an alternative to the conventional models.