Escherichia coli was the prevailing bacterial infections among those which were isolated from patients with UTI. Certain forms of bacterial infections inclined to be extra common in diabetic patients than others and other infections may be more severe in people with diabetics than in non diabetics.

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Klebsiella oxytoca was grown in batch cultures using a 2-chlorobenzoic acid (2-CBA) containing minimal salt medium. 2-CBA was the sole source for carbon and energy. 1 H NMR and electron-impact mass spectra results suggested an ortho-cleavage pathway of the 2-CBA degradation. An unstructured model with substrate inhibition was successfully applied to the 2-CBA experimental data at different concentrations. The maximum specific growth rate, saturation constant, and substrate inhibition constant were found to be 0.8573 h -1 , 10.441 mM, and 50.95 mM, respectively. All carbon sources exhibited different growth kinetics and caused an increase in bacterial growth several times (2-4 fold). Glucose, sorbitol, and fructose at 0.2% concentration, as additional carbon sources, decreased the initial degradation rates of 2-CBA at ranges of 20-70% lower than that of the control. At the same time, lactose, maltose, and sucrose led to the increase in the 2-CBA biodegradation. Nitrogen sources (except L-prolin) caused an inhibition in 2-CBA degradation by 55, 60, and 33% for yeast extract, casein, and trypton, respectively. The data showed that different initial (inocula) densities affected the induction time for 2-CBA degradation and resulted in the different degradation rates by concentrationdependent manner. 2-CBA degradation was optimally achieved at a 37°C incubation temperature and a pH of 7.0.

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