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Introduction 3. Hormonal regulation of insect metamorphosis 3.1. Larval molting and pupal metamorphosis 3.2. Alterations of cells during metamorphosis 4. Transcriptional regulation of cuticle protein genes during metamorphosis 4.1. CP genes expressed pre-pupal stage showed six distinctive developmental patterns 4.2. Responsiveness of CPs to the ecdysone pulse in vitro is reflected in the developmental profile 4.3. Prepupal CP genes showed different ecdysone responsiveness, resulting in different developmental profiles of ecdysone-responsive transcription factors 5. Summary and perspective 6. Acknowledgments 7. References