Transcriptional regulation of cuticular genes during insect metamorphosis

Ms, Ali; Takaki, Keiko

doi:10.2741/4796

Cited by 8 publications

(1 citation statement)

References 41 publications

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“…A plethora of physiological processes has been reported to be affected by the knockdown of cuticle protein, including locomotion, mechanical support, sensory perception, molting, and resistance response [ 44 ]. The molting process also involves a principal hormone in the insects, i.e., the juvenile hormone (JH) binding protein [ 47 ]. The transcriptome sequencing data indicated that in WBPH, there is a >6.0-fold upregulation of this hormone, clearly indicating that resistant hosts trigger changes in the developmental processes in these insects, specifically those related to molting and metamorphosis.…”

Section: Discussionmentioning

confidence: 99%

Expression Analysis Reveals Differentially Expressed Genes in BPH and WBPH Associated with Resistance in Rice RILs Derived from a Cross between RP2068 and TN1

Anand,

Divya,

Mazumdar-Leighton

et al. 2023

IJMS

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BPH (brown planthopper) and WBPH (white backed planthopper) are significant rice pests that often co-occur as sympatric species and cause substantial yield loss. Despite their genetic similarities, different host-resistance genes confer resistance against these two hoppers. The defense mechanisms in rice against these pests are complex, and the molecular processes regulating their responses remain largely unknown. This study used specific recombinant inbred lines (RILs) derived from a cross between rice varieties RP2068-18-3-5 (BPH- and WBPH-resistant) and TN1 (BPH- and WBPH-susceptible) to investigate the mechanisms of interaction between these planthoppers and their rice hosts. WBPH and BPH were allowed to feed on specific RILs, and RNA-Seq was carried out on WBPH insects. Transcriptome profiling and qRT-PCR results revealed differential expression of genes involved in detoxification, digestion, transportation, cuticle formation, splicing, and RNA processing. A higher expression of sugar transporters was observed in both hoppers feeding on rice with resistance against either hopper. This is the first comparative analysis of gene expressions in these insects fed on genetically similar hosts but with differential resistance to BPH and WBPH. These results complement our earlier findings on the differential gene expression of the same RILs (BPH- or WBPH-infested) utilized in this study. Moreover, identifying insect genes and pathways responsible for countering host defense would augment our understanding of BPH and WBPH interaction with their rice hosts and enable us to develop lasting strategies to control these significant pests.

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Section: Discussionmentioning

confidence: 99%

Expression Analysis Reveals Differentially Expressed Genes in BPH and WBPH Associated with Resistance in Rice RILs Derived from a Cross between RP2068 and TN1

Anand,

Divya,

Mazumdar-Leighton

et al. 2023

IJMS

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Chlorbenzuron downregulated HcLCP‐17 expression by depressing two 20E‐responsive transcription factors Br‐C and βFTZ‐F1 in Hyphantria cunea (Lepidoptera: Erebidae) larvae

Zhao,

Zhang,

Zou

et al. 2024

Pest Management Science

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BACKGROUNDCuticular proteins (CPs) play essential roles in forming cuticular structures in insects. However, the specific functions and regulatory mechanisms of CPs remain largely unexplored. In this study, the Larval cuticular protein 17 (HcLCP‐17) gene was identified from Hyphantria cunea, a highly destructive and polyphagous forest pest. To investigate the role of HcLCP‐17 in cuticular function and transcriptional regulation mediated by 20E‐responsive transcription factors (ERTFs), we employed RNA interference (RNAi) and yeast one‐hybrid assay techniques. Additionally, we examined the molecular mechanism by which chlorbenzuron, a type of benzoylphenylurea (BPU) that functions as a chitin synthesis inhibitor (CSI), affects the 20E signaling pathway and ultimately regulates HcLCP‐17 expression.RESULTSHcLCP‐17 encodes a polypeptide consisting of 393 amino acids, which includes a chitin‐binding domain. Silencing HcLCP‐17 resulted in a disturbance in the structural organization of the larval cuticle and a notable reduction in chitin levels. HcLCP‐17 expression was controlled by the interaction between Broad‐Complex (Br‐C) and beta Fushi Tarazu Factor‐1 (βFTZ‐F1) with its promoter fragment. Furthermore, the inhibitory effect of chlorbenzuron on HcLCP‐17 expression was found to be potentially mediated by Br‐C and βFTZ‐F1.CONCLUSIONThe study presents a novel mode of action for the 20E signaling pathway in regulating the expression of CPs and reveals the potential mode‐of‐action of BPUs in insect cuticles. These findings provide a theoretical basis for future utilization of LCP‐17 as a pesticide target making a significant contribution to the development of effective pest management strategies. © 2024 Society of Chemical Industry.

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Vibrio cholerae high cell density quorum sensing activates the host intestinal innate immune response

et al. 2022

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Transcriptional regulation of cuticular genes during insect metamorphosis

Cited by 8 publications

References 41 publications

Expression Analysis Reveals Differentially Expressed Genes in BPH and WBPH Associated with Resistance in Rice RILs Derived from a Cross between RP2068 and TN1

Expression Analysis Reveals Differentially Expressed Genes in BPH and WBPH Associated with Resistance in Rice RILs Derived from a Cross between RP2068 and TN1

Chlorbenzuron downregulated HcLCP‐17 expression by depressing two 20E‐responsive transcription factors Br‐C and βFTZ‐F1 in Hyphantria cunea (Lepidoptera: Erebidae) larvae

Vibrio cholerae high cell density quorum sensing activates the host intestinal innate immune response

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