This study was performed to investigate the effect of dietary supplementation with Aloe vera (AV) powder and synbiotic as growth promoter agents on performance, gut morphology, immune responses, hematology and serum biochemistry in broilers. A total of 240-day-old male broiler chicks (Ross 308) were randomly assigned to six treatments with four replicates. Birds were offered either a corn-soybean meal basal diet (control) or the basal diet supplemented with 200 mg/kg virginiamycin (VM), 1 g/kg synbiotic (Syn), 2.5 g/kg AV (AV1), 5.0 g/kg AV (AV2) or 7.5 g/kg AV (AV3). Chickens fed any of the diets, except diet AV1, exhibited better feed conversion ratios at the 14-28 day period and higher average daily gain and duodenal villus height/crypt depth ratio at 42 days than those fed the control diet. Synbiotic supplementation caused a marked increase in the serum antibody titer against infectious bursal disease and infectious bronchitis vaccines. Feeding diet AV3 significantly increased red blood cell count and hemoglobin concentration, and decreased serum triglyceride level compared to the control group. The results suggested that dietary inclusion of 5 and 7.5 g/kg AV, similar with synbiotic supplementation, can be applied as effective alternatives to in-feed antibiotics for broiler diets.