The aim of this study was to examine the anticancer activities and phytochemical profiles of Dicranum scoparium against HeLa cell lines. The bio-guided fractionation studies of dichloromethane extract have high antiproliferative activities. Fractions 7, 9, 19, 20 are rich source of unsaturated fatty acids, and- in the case of Fr-19 may improve the antiproliferative activities as well as increase the unsaturated fatty acid content. The effect of proliferative activities in hexane extract can be attributed to the saturated fatty acid composition of D. scoparium. The Fr-9 exhibited strong antiproliferative activity at concentrations of 100 and 50 μg mL(-1) compared to 5-FU. The fractions of 7, 9, 19 and 20 from dichloromethane extracts exhibited antiproliferative activities at a concentration of 100 μg mL(-1). The HPLC-TOF/MS studies gave nine compounds from the most active fraction of dichloromethane at concentrations of 250 and 100 μg mL(-1). The lower activities were obtained from the fractions including steroid derivatives.

No abstract

No abstract

No abstract

Mint has been used extensively in traditional medicines, spices, and herbal teas throughout the world. Isolation of flavonoids from Mentha spicata and quantification of these compounds in selected clones of Turkish mints landraces were achieved. M. spicata was dried in shade and then boiled in water. After filtration, the aqueous extract was partitioned with ethyl acetate. The solvent was removed under reduced pressure to yield the extract, subjected to silica gel column chromatography to isolate 5-demethyl sinensetin (1), hesperidin (2), didymin (3), and linarin (4), the structures of which were elucidated by 1D NMR, 2D NMR, and LC-TOF/MS. The isolated compounds were employed as standard flavonoids to determine the quantitative variations in cultivated Turkish mint landraces belonging to Mentha × piperita L., M. spicata L., and M. × villosonervata Opiz by HPLC. The quantities of hesperidin (2), didymin (3), and linarin (4) in M. spicata were found as 11.83, 3.85, and 42.21 mg/g dried plant weight (DW), respectively, while that of 5-demethyl sinensetin (1) in M. villosonervata was 2.9 mg/g DW. Consisting of pharmaceutically valuable compounds, M. spicata and cultivated Turkish mint landraces could be a source of 5-demethyl sinensetin (1), hesperidin (2), didymin (3), and linarin (4).