Determination of Antiproliferative Activities of Volatile Contents and HPLC Profiles of Dicranum scoparium (Dicranaceae, Bryophyta)

Abay, Gökhan; Altun, Muhammed; Koldaş, Serkan; Tüfekçi, Ali Rıza; Demirtaş, İbrahim

doi:10.2174/1386207318666150305112504

Cited by 37 publications

(36 citation statements)

References 17 publications

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“…Anti-proliferative activity measurements were performed according to the method described elsewhere (Abay et al, 2015;Ökten et al, 2015). 50 µL DMEM were added into each well of E-Plate 96.…”

Section: Cell Proliferation Assaymentioning

confidence: 99%

HPLC analysis, anti-oxidant activity of Genista ferox and its anti-proliferative effect in HeLa cell line

Bencherchar

Demirtaş

Altun

et al. 2017

Bangladesh J Pharmacol

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Research ArticleHPLC analysis, anti HPLC analysis, anti HPLC analysis, anti---oxidant activity oxidant activity oxidant activity of of of Genista ferox Genista ferox Genista ferox and its anti and its anti and its anti---proliferative effect proliferative effect proliferative effect in in in HeLa HeLa HeLa cell cell cell line line line BJP IntroductionPlants used in ancient times as medicines to alleviate symptoms of various ailments (Saeed et al., 2012). In spite of the great progress in modern medicine in recent decades, but the herbal medicine still make an important contribution in the health care. Many medicinal and aromatic plants contain huge amounts of antioxidants such as polyphenols. These substances can have an important role in the absorption and neutralize free radicals, and the extinction of the shirt and triple oxygen or peroxide decomposing. Many of these phytochemicals have anti-oxidant capabilities so they contribute significantly to the fight against many human diseases and thus contribute to the reduction of mortality (Djeridane et al., 2006). Phenolic compounds such as flavonoids and phenolic acids have different biological effects, such as the effects of anti-atherosclerotic, anti-inflammatory and anti-cancer, as a result of the anti-oxidant activity (Krishnaiah et al., 2011).The Fabaceae family contains approximately 700 genera, in Algeria there are about 53 genera and 337 species (Quezel and Santa, 1963). Genista genus has about 150 species in Europe and the Mediterranean region (RE, 1987). A literature survey shows that this genus is a good source of phenolic compounds, in particular isoflavonoids, which are known for their diverse biological activities. The recent studies on the species of the Genista genus showed pharmacological interest (Belle et al., 1995;Herrera et al., 1992).In the present study, the qualitative and quantitative analysis, the identification and quantification of phenolic acids and flavonoids in chloroform, ethyl AbstractThe prevention and treatment of the cancer using plants have attracted increasing interest. The present study was aimed to determine the phenolic compounds of Genista ferox using HPLC-TOF/MS and the anti-oxidant activity associated with anti-cancer activity against human cervical adenocarcinoma (HeLa) cell line. Total anti-oxidant capacities of different extracts of G. ferox were assessed by DPPH assay, and their total phenolic and flavonoids contents measured by Folin-Ciocalteu and aluminum trichloride assays. The amounts of total phenolic (105.2 ± 0.6 -308.5 ± 5.7 mg/g) of extract measured as gallic acid equivalent and flavonoids (8.1 ± 0.1 -124.0 ± 0.7 mg/g) of extract measured as quercetin equivalent varied from chloroform to n-butanol extract of the two parts of the plant (leaf and stem). The ethyl acetate extract of G. ferox exhibited the most powerful effect on the DPPH scavenging activity with 94% from the leaf and 93% from the stem, while the chloroform extract from the leaf exhibited the most effective anti-proliferative activity against H...

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Section: Cell Proliferation Assaymentioning

confidence: 99%

HPLC analysis, anti-oxidant activity of Genista ferox and its anti-proliferative effect in HeLa cell line

Bencherchar

Demirtaş

Altun

et al. 2017

Bangladesh J Pharmacol

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“…Injection volume was 10 µl. Phenolic compounds analysis was carried out as described by [9], using an Agilent Technologies 1260 Infinity HPLC System coupled with 6210 Time of Flight (TOF) LC/MS detector. The separation was carried out on ZORBAX SB-C18 (4.6 x100 mm, 3.5μm) column.…”

Section: Analysis Of Phenolic Compound By Hplc-tof/msmentioning

confidence: 99%

Antioxidant and Anti-Inflammatory Activities of Rubus Fruticosus and Zizyphus Vulgaris Methanol Extracts

Meziti

Bouriche

Hichem

et al. 2017

Int J Pharm Pharm Sci

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Objective: This report is an attempt to study the phenolic composition of Rubus fruticosus (RFE) and Zizyphus vulgaris (ZVE) methanol extracts and evaluate their antioxidant and anti-inflammatory effects in-vitro and in-vivo. Methods:Total phenolic and total flavonoids contents of extracts were determined by spectrophotometric methods. Phenolic compounds were identified by HPLC-TOF/MS. The antioxidant activities were evaluated in vitro using DPPH, ABTS and FRAP assays. The effect of RFE and ZVE on DNA cleavage induced by H2O2 Results:The phytochemical analysis of these extracts showed that RFE possesses higher polyphenolic and flavonoid content than ZVE. in the same way RFE exerted the highest antioxidant capacity with IC UV-photolysis was also investigated. The antioxidant effect of RFE and ZVE was tested in vivo using the blood total antioxidant capacity test in mice. On the other hand, the anti-inflammatory activity was assessed in vivo using two models of acute inflammation ear edema and vascular permeability. 50 value of 14 µg/ml in DPPH assay, 1.58 mmol of Trolox E/mg extract and 3.39 of mmol FesO4/mg extract in ABTS, and FRAP assay respectively. The studied extracts showed a concentration-dependent protective effect on DNA cleavage induced by H2O2 UV-photolysis. The daily oral administration of 200 mg/kg of RFE or ZVE during three weeks showed an improvement of the blood total antioxidant capacity; the HT50 Conclusion:The results obtained in this investigation showed that RFE possesses strong antioxidant and anti-inflammatory potential in comparison with ZVE, which may be attributed to the presence of polyphenolic phytoconstituents. values were151.45 min and 146.72 min for the groups treated with RFE and ZVE, respectively versus 122.5 min for the control group. The topical application of 2 mg/ear of RFE inhibited the croton oil-induced ear edema by 75.72%, while the inhibition exerted by ZVE was 64.24%. These inhibitions were higher than that of indomethacin, used as a reference. Moreover, the oral administration of 400 mg/kg of RFE inhibited significantly (33.57%) acetic acid induced vascular permeability in mice. However, this effect was lower than this of indomethacin. The inhibition effect exerted by ZVE was not significant.

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“…A previous report on Dicranum scoparium proposed that fractions of the plant contained phytol acetate and proffered antiproliferative effects on HeLa cancer cells [42]. Since phytol acetate was also present in F1, F3 and F4, the findings might indicate that phytol acetate did not have high impact on antiproliferation of cancer cells.…”

Section: No Compoundsmentioning

confidence: 84%

Antiproliferative, Antioxidative and Compounds Identification from Methanolic Extract of Passiflora Foetida and Its Fractions

Sul’ain¹,

Sisin²,

Abdullah³

2017

JAPLR

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Determination of Antiproliferative Activities of Volatile Contents and HPLC Profiles of Dicranum scoparium (Dicranaceae, Bryophyta)

Cited by 37 publications

References 17 publications

HPLC analysis, anti-oxidant activity of Genista ferox and its anti-proliferative effect in HeLa cell line

HPLC analysis, anti-oxidant activity of Genista ferox and its anti-proliferative effect in HeLa cell line

Antioxidant and Anti-Inflammatory Activities of Rubus Fruticosus and Zizyphus Vulgaris Methanol Extracts

Antiproliferative, Antioxidative and Compounds Identification from Methanolic Extract of Passiflora Foetida and Its Fractions

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