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Background: Intraoperative radiation therapy (IORT) is a novel approach to breast cancer (BC) treatment. Objectives: In this study, we compared the cellular and molecular effects of IORT-treated post-lumpectomy wound fluid (seroma) at the point of IOeRT versus IOxRT on the BC cell line. Methods: Immortalized human BC cell lines: MCF-7, MDA-MB-231, and MCF10 were incubated with seroma from 3 groups of patients (as a pilot study). The first group received Intraoperative electron radiation therapy (IOeRT, Boost dose=12Gy), the second one received IOeRT (Radical dose=21Gy), and the third group was prescribed Intraoperative x-ray radiation therapy (IOxRT, X-ray=20Gy). Cellular and molecular tests were used to investigate how cells are influenced by the IORT-treated seroma. Results: We evaluated the effects of dose-time and source-dependent IORT-treated seroma on BC cell lines. In this study, we observed that IOxRT-treated seroma has the most significant effects on the reduction of proliferation, induced cell cycle arrest, and apoptosis. Furthermore, inhibited migration and invasion of BC cell lines were compared to IOeRT -treated seroma. Conclusions: Although this is a pilot study, we suggest that at 24 h, the IORT (specifically IOxRT)-treated seroma may play an important protective role in the breast tumor bed, which is followed by local recurrence decreases.

Introduction: Due to the high prevalence of low-risk breast cancer (BC) and the side effects affecting families in Iran, related researches are necessary. BC is one of the main leading causes of death in women, causing 40,000 to 50,000 deaths per year. Therefore, the recognition of prognostic factors, biomarkers and preventive factors can be useful for selecting appropriate therapies and clinical decisions. Materials and Methods: According to Keywords: Breast Cancer, Biomarkers, Early detection and cancer Stem cell that utilized the valid databases, such as: Scopus, NCBI and Elsevier. The papers collected and then was ranking based on appropriate criteria. Results: In this study, according to key words in the field of Biomarkers involved in Breast Cancer 65 articles were selected. After completing the final analysis, 15 studies from other research more relevant to their subject, were assessed. Conclusions: The heterogeneous and difficult nature of the BC is becoming a leading condition to define, in accordance with its growing incidence and poor. According to of Results, identify CD44 as a biomarker that is important in the early detection of Breast Cancer.

Background Radiotherapy (RT) is recommended to all patients undergoing Breast Conserving Surgery (BCS). Two strategies can be applied to irradiation, External Beam RT (EBRT) in addition, Intraoperative Radiation Therapy (IORT). The aim of this study was to introduce a protein biomarker panel related to molecular function under IORT. Methods Six Breast Cancer (BC) patients as a pilot study were treated by 12 Gy (Boost dose) and 21 Gy (Radical dose). Samples tissue included Margin before IORT (MB), and Margin 24 hours After IORT (MA24 h). Isobaric Tag for Relative and Absolute Quantitation (iTRAQ) was performed to study proteomic of IORT-treated tumor bed. Results We classified 110 differentially expressed proteins (DEPs) as a protein biomarker panel by mapping the annotated coding region sequences to the reference canonical pathways in the KEGG database. Conclusion Our findings indicate that the DEPs may be key proteins in IORT-treated tumor bed and may serve as potential Effective biomarkers under IORT.