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Introduction: The Expanded Program on Immunization (EPI) is meant for thecontrol of common infectious microorganism. Objectives: To know the awareness amongthe mothers and its relation with their education. Study Design: Cross-sectional study.Setting: Pediatric unit, Civil Hospital Bahawalpur. Period: June 15, 2015 to August 31, 2015.Methods and Material: The mothers were interviewed by the researchers using a structuredquestionnaire. Results: There were 100 mothers included in this study. Their mean age± SDwas 28.02± 7.468 years. Among the studied mothers 20 (20%) were having at least secondaryschool certificate. There were 88 (88%) mothers who were house wives. Only 3 (3%) mothersanswered the correct number of vaccines used in EPI program. The awareness about individualvaccine was in the range of 28%-33% except in polio it was 76% and in measles it was 61%.The 40 (40%) mothers answered that EPI vaccines are safe and 37(37%) replied that theseare effective. The mother’s high education status was associated with better awareness aboutEPI. Conclusion: There is lack of knowledge among mothers about EPI vaccination and themother’s high education status is associated with better awareness about EPI.