Summary Every protein family has a set of characteristic secondary structures. However, due to individual variations, a single structure is not enough to represent the whole family. OverProt can create a secondary structure consensus, showing the general fold of the family as well as its variation. Our server provides precomputed results for all CATH superfamilies and user-defined computations, visualized by an interactive viewer, which shows the secondary structure element type, length, frequency of occurrence, spatial variability and β-connectivity. Availability and implementation OverProt Server is freely available at https://overprot.ncbr.muni.cz. Supplementary information Supplementary data are available at Bioinformatics online.

Due to their paramount importance, near-ubiquitous presence, and complex nature, nicotinic acetylcholine receptors (nAChRs) have remained the focus of intensive research for over 50 years. The vast amount of knowledge accumulated on the topic has become extremely difficult to navigate. NAChRDB addresses this challenge by providing web-based, real-time access to curated residue-level functional annotations of neuromuscular nAChRs with interactive 3D visualization and sequence alignment. NAChRDB provides systematic access to experimental observations and predictions from computational studies reported in the literature or performed specifically to complement current knowledge, which allows new findings to be interpreted in a more holistic context, both from a structural and a functional perspective. NAChRDB aims to serve as an invaluable resource for identifying gaps in knowledge and for guiding discovery through structural and molecular biology experiments, especially when exploring the allosteric mechanisms underlying neuromuscular nAChR function and pathology. Availability and Implementation: NAChRDB is freely available online, with a self-explanatory interface and useful tool tips (https://crocodile.ncbr.muni.cz/Apps/NAChRDB/). No installation or user registration is required. NAChRDB content is stored in .json format, queried using Python, and rendered in browser using Javascript and WebGL (LiteMol). NAChRDB is highly responsive and accessible through any modern Internet browser on desktop and mobile devices.

Nicotinic acetylcholine receptors (nAChRs) comprise a large and ancient family of allosteric ion channels mediating synaptic transmission. The vast knowledge about nAChRs has become difficult to navigate. NAChRDB is a web-accessible resource of curated residue-level functional annotations of neuromuscular nAChRs. Interactive three-dimensional (3D) visualization and sequence alignment give further context to this rich and growing collection of experimental observations and computational predictions. NAChRDB is freely available at , with interactive tutorials and regular updates to the content and web interface. No installation or user registration is required. NAChRDB is accessible through any modern internet browser on desktops and mobile devices. By providing immediate and systematic access to practical knowledge gained through decades of research, NAChRDB represents a powerful educational tool and helps guide discovery by revealing gaps in current knowledge and aiding the interpretation of results of molecular and structural biology experiments or computational studies.