The objective of this study was to evaluate the efficiency of the enzyme β-1, 3-glucanase and the mechanisms of action produced by yeasts in the biological control ofgreen mold in post-harvest oranges of the pear variety. The Fully Casualized Delineation(FCD) was used. The results were submitted to analysis of variance (ANOVA), in triplicate,compared with Tukey's test at a 5% probability level (p < 0.05), using the statisticalsoftware Sisvar. It was found that the killer activity was observed in 85.7% of the yeastsevaluated. The yeast Candida peltata URM4681 showed better production of β-1.3-glucanase, between 1.071 and 5.422 µg mL -1 . A factorial delineation 2 3 was used in amedium composed of yeast extract, soybean extract and meat extract to increaseenzymatic production, reaching an increase of 4.351 times comparing the assay of lowerand higher activity. The in situ assays showed a reduction in the number of infected fruitstreated with the enzymatic extract, being on average 78.5% when compared with thecontrol treatment (distilled water). As for sporulation, the enzymatic extract, which had anaverage of 8.4 spores, did not differ statistically from the IMZ and TBZ fungicides, both in apreventive and curative manner. Regarding the severity of the disease, it was noted thatthe fruits treated with enzymatic extract, had an average damage of 7.57 %, also notdiffering statistically from fungicides, both preventive and curative. Our studies suggestthat C. peltata URM4681, makes this a good candidate for biocontrol against green mold.
A adição de cultura probiótica confere potencial biofuncional aos alimentos, devido à capacidade de promover a saúde e aos efeitos bioquímicos e fisiológicos no organismo. O objetivo foi produzir iogurte com lactose e sem lactose com adição de Bacillus subtilis e avaliar o potencial antioxidante de peptídeos solúveis em água durante o período de armazenamento. Para a produção de iogurte, foi utilizado um arranjo fatorial simples para avaliar dois tipos de leite com adição da cultura probiótica contendo B. subtilis UFPEDA 86. As frações foram avaliadas (pré-digestão e pós-digestão) quanto ao potencial antioxidante de eliminação do radical do radical 2 Ácido 2'-azino-bis (ácido 3- etilbenzotiazolina -6-sulfônico) (ABTS), do 2,2'-difenil-1-picril-hidrazil (DPPH) e quelação de cobre e ferro. Na avaliação dos resultados, as frações obtidas das diferentes combinações de leite, cultura probiótica e dias de armazenamento apresentaram potencial antioxidante e capacidade quelante; as atividades apresentaram aumento significativo após o processo de digestão. Além disso, os resultados demonstraram que o B. subtilis pode ser empregado na produção de bio-iogurte com potencial antioxidante.
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