The Federal District of Brazil (FD) has the lowest Phlebotominae species richness in the country's Midwestern Region. Some of these species have been reported as Leishmania vectors. This study updates the list of sand flies in the FD by sampling 4 gallery forests. Using HP light traps and Shannon traps, 1,209 sand flies were captured and 16 species were identified. The most frequent species was Bichromomyia flaviscutellata (Mangabeira, 1942) (N = 668), followed by Psathyromyia pradobarrientosi (Le Pont, Matias, Martinez & Dujardin, 2004) (N = 285). Brumptomyia guimaraesi , Micropygomyia ferreirana (Martins, Barretto & Pellegrino, 1956), Pa. pradobarrientosi, Br. brumpti (Larrousse, 1920, and Evandromyia bourrouli are reported for the first time in the FD. Sand flies richness in the FD (35 spp.) is relevant when compared to that of the state of Goiás (47 spp.), a state 59 times larger than the FD.