An updated list of sand flies (Diptera, Psychodidae, Phlebotominae) in the Federal District of Brazil

Rapello, Aline; Andrade, Andrey José de; Rocha, Douglas de Almeida; Ferreira, Jônatas C. B.; Timbó, Renata Velôzo; Obara, Marcos Takashi; Gurgel-Gonçalves, Rodrigo

doi:10.15560/14.1.213

Cited by 5 publications

(2 citation statements)

References 29 publications

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“…We did not capture phlebotomines in the gallery forest adjacent to the zoo. We expected to capture sandflies in this area because they are frequent in gallery forests of the FD (Ferreira, Macedo, et al, ; Rapello et al, ). The absence of Leishmania ‐infected sandflies may be directly related to the low number of specimens captured and the low rate of infection by trypanosomatids in the studied area (Ferreira, Minuzzi‐Souza, et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…cruzi in the forest adjacent to the Brasília Zoo (Gurgel‐Gonçalves et al, ). Leishmania infantum ‐infected dogs have also been confirmed in FD (Cardoso et al, ), where sandflies are frequent in gallery forests (Ferreira, Minuzzi‐Souza, et al, ; Rapello et al, ) and houses (Carvalho, Bredt, Meneghin, & Oliveira, ). Studies of trypanosomatid‐infected mammals and vectors (triatomines and sandflies) in zoos are important for the establishment of surveillance and control measures for leishmaniasis and Chagas disease, including valuable information for animal health and management strategies such as translocations.…”

Section: Introductionmentioning

confidence: 95%

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Trypanosomatid infections in captive wild mammals and potential vectors at the Brasilia Zoo, Federal District, Brazil

Reis

Minuzzi-Souza

Neiva

et al. 2019

Veterinary Medicine & Sci

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Background: Conservation projects in zoos may involve translocation of captive animals, which may lead to pathogen spread. Neotropical mammals are important hosts of Trypanosoma cruzi and Leishmania spp. the etiological agents of Chagas disease and Leishmaniasis respectively. Studies of trypanosomatid-infected mammals and vectors (triatomines and sandflies) in zoos are important for the establishment of surveillance and control measures.Objectives: We investigated trypanosomatid infections in captive wild mammals, triatomines and sandflies at the Brasília Zoo. Methods:We collected triatomines during active bimonthly surveys, sampled sandflies using light-traps and obtained blood samples from 74 mammals between 2016 and 2017. We used quantitative PCR to detect trypanosomatids in vectors and mammals. Results:We found a colony of 19 Panstrongylus megistus in the porcupine unit and detected T. cruzi infections in five bugs. We captured 17 sandflies of four speciesincluding Nyssomyia whitmani and Lutzomyia longipalpis, but no Leishmania infection was detected. qPCR detected 50 T. cruzi-infected mammals belonging to 24 species and five groups of mammals (Carnivora, Cetartiodactyla, Perissodactyla, Pilosa and Primates); Leishmania DNA was detected in 23 mammals from 15 species, mainly carnivores. We detected trypanosomatid infections in 11 mammals born at the Brasília Zoo. Conclusions:Our results suggest vector-borne transmission of T. cruzi among maned wolves; measures to reduce the risk of new infections should therefore be taken. We also report sandfly presence and Leishmania-infected mammals at the Brasília Zoo. Translocation of wild mammals in and out of the Brasília Zoo should consider the risk of T. cruzi and Leishmania spread. K E Y W O R D S Conservation, Leishmania, Translocation, Trypanosoma, Zoonosis | 249 REIS Et al.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Trypanosomatid infections in captive wild mammals and potential vectors at the Brasilia Zoo, Federal District, Brazil

Reis

Minuzzi-Souza

Neiva

et al. 2019

Veterinary Medicine & Sci

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High molecular prevalence of Leishmania in phlebotomine sand flies fed on chicken blood in Brazil

Ferreira

Timbó

Minuzzi-Souza

et al. 2018

Veterinary Parasitology

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Leishmaniases are endemic in Brazil, where Leishmania infantum has been detected in humans, dogs, cats, and phlebotomine vectors. Monitoring synanthropic vector populations is critical for leishmaniasis control-surveillance in such transmission-prone areas. Here, a suite of molecular approaches were used to assess Leishmania infection prevalence and to identify blood-meal sources in a large sample of sand flies collected in anthropic environments of a Leishmania-transmission area in Mato Grosso do Sul State (Rio Verde de Mato Grosso municipality), Central-West Brazil. We sampled sand flies monthly (January-June 2014 and 2016) in one peri-domestic site within each of six neighborhoods with recent records of human visceral and/or tegumentary leishmaniasis. kDNA-qPCR plus rDNA ITS-sequencing were used to detect and identify Leishmania in pooled female sand flies. Individual engorged females (n = 58) were used for blood-meal analyses through High-Resolution Melting (HRM) targeting the mtDNA cytb gene. Overall, 90.5% of 420 CDC trap-nights yielded vectors, for a total catch of 24,989 sand flies. We sub-sampled and identified 3088 sand flies of 12 species, including 2775 Lutzomyia longipalpis (the most abundant species at all sampling sites) and 297 Nyssomyia whitmani. Female sand flies (n = 1261) were grouped in 159 pools, of which 92 Lu. longipalpis (minimum infection rate [MIR] 8%) and 7 Ny. whitmani pools (MIR 7%) were Leishmania kDNA-positive. Most positive Lu. longipalpis were collected in the 2016 rainy season. Sequencing confirmed L. infantum in Lu. longipalpis samples. HRM analyses identified chicken DNA in 57 sand flies (98.3%), 37 of which were Leishmania DNA-positive (64.9%); human blood was found in just one (Leishmania-negative) female. Our data show ongoing risk of L. infantum transmission to humans in the study area, where Leishmania-infected sandfly vectors are common and heavily rely on chicken blood in the peri-domestic environment.

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New Records of Phlebotomine Sand Flies (Diptera: Psychodidae) From the Western Brazilian Amazon and the Description of the Female of Pintomyia fiocruzi

Costa

Júnior

Pessoa

et al. 2020

Journal of Medical Entomology

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Abstract An entomological survey was conducted in several localities of Rondônia State, and new records were obtained for seven sand fly species: Evandromyia apurinan Shimabukuro, Figueira & Silva, 2013, Evandromyia carmelinoi (Ryan, Fraiha, Lainson & Shaw, 1986), Micropygomyia echinatopharynx Andrade-Filho, Galati, Andrade & Falcão, 2004, Nyssomyia urbinattii Galati & Galvis, 2012, Pintomyia duckei Oliveira, Alencar & Freitas, 2018, Psathyromyia pradobarrientosi (Le Pont, Matias, Martinez & Dujardin, 2004), and Sciopemyia vattierae (Le Pont & Desjeux, 1992). Herein, we also describe the female of Pintomyia fiocruzi Pereira Júnior, Pessoa, Marivalva & Medeiros, 2019. The female has spermathecae with a detached apical ring similar to the spermathecae of Pintomyia serrana (Damasceno & Arouck, 1949), Pintomyia odax (Fairchild & Hertig, 1961), Pintomyia ottolinai (Ortiz & Scorza, 1963), and Pintomyia robusta (Galati, Cáceres & Le Pont, 1995). Here, we provide characters to separate Pi. fiocruzi from Pi. serrana and Pi. odax. Pintomyia ottolinai has a trans-Andean distribution and Pi. robusta has not been found in Brazil and in our studies, we associated the females found in this study with Pi. fiocruzi males found at the same collection sites. Our findings highlight the importance of entomological fauna surveys and demonstrate the need for taxonomists to study species diversity in Rondônia. This study increases the number of sand fly species recorded in Rondônia to 143.

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An updated list of sand flies (Diptera, Psychodidae, Phlebotominae) in the Federal District of Brazil

Cited by 5 publications

References 29 publications

Trypanosomatid infections in captive wild mammals and potential vectors at the Brasilia Zoo, Federal District, Brazil

Trypanosomatid infections in captive wild mammals and potential vectors at the Brasilia Zoo, Federal District, Brazil

High molecular prevalence of Leishmania in phlebotomine sand flies fed on chicken blood in Brazil

New Records of Phlebotomine Sand Flies (Diptera: Psychodidae) From the Western Brazilian Amazon and the Description of the Female of Pintomyia fiocruzi

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