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SUMMARYThe production, purification and properties of the 'A' antigen induced by Marek's disease virus in tissue culture were investigated. It was found that although virus multiplied on passage in chick kidney, chick embryo and duck embryo cells the antigen was produced only in chick kidney and duck embryo cultures. The results suggested that the 'A' antigen produced by duck embryo cells was a glycoprotein having a mol.wt, in the range 7o ooo to 9o ooo and was heterogeneous in charge (pI 4"5 to 5"5)-A 2o-fold purification was achieved by electrophoresis in 5 ~o acrylamide gel with a recovery of 45 ~. An equally good purification was also possible by chromatography on DEAE-Sephadex A5o and Sephadex G2oo, although the recovery was only 20 ~o in this case.

Proteose-peptone-activated mouse macrophages can prevent productive infection by herpes simplex virus in neighboring cells in vitro whether or not those cells belong to the same animal species. The effect does not require contact between the macrophages and the infected cells, may be prevented by adding extra arginine to the medium, and may be reversed when extra arginine is added 24 h after the macrophages. Arginase activity was found both intracellularly and released from the macrophages. The extracellular enzyme is quite stable; 64% activity was found after 48 h of incubation at 37°C in tissue culture medium. No evidence was found that the inefficiency of virus replication in macrophages was due to self-starvation by arginase. As might be predicted macrophages can, by the same mechanism, limit productive infection by vaccinia virus.

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