The fruit oil of Carum copticum showed the presence of eleven components with carvacrol (45.20%) and δ−cymene (41.98%) as the major constituents by GC-MS analysis. The essential oil exhibited considerable in vitro antimicrobial activity with most of the gram-positive and gram-negative bacteria tested and the results are comparable with the standard bactericide. The pure oil inhibited the growth of Phomopsis destructum, Aspergillus niger and A. flavus.

In recent years there is a spurt in the interest regarding survival of Ayurvedic forms of medication. In the global perspective, there is a shift towards the use of medicine of herbal origin, as the dangers and the shortcoming of modern medicine have started getting more apparent, majority of Ayurvedic formulation are prepared from herbs. Syrup is very popular dosage form of cough and cold medications, ease of patient compliance. The objective of this study is to develop a herbal cough syrup and to check the anti bacterial activity of the extract of seeds of Hedge mustard used for the formulation of herbal cough syrup against different bacteria (Staphylococcus aureus, E.coli, Salmonella sp., Pseudomonas aeruginosa , B.subtilis), and to evaluate the physicochemical parameter of cough syrup as well. The cough syrup formulated with simple syrup 66.67% w/v as sugar base. Quality of final herbal syrup was evaluated with different parameters: physical appearance (colour, odour, taste), pH and turbidity. The formulated syrup under gone stability studies and microbial test for 72 hours, no turbidity was observed and no microbial growth was seen.

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Development of green nanotechnology is generating interest of researchers toward ecofriendly biosynthesis of nanoparticles. In this study, biosynthesis of stable copper nanoparticles were done using Ficus Racemosa leaf extract. First we prepared leaf extract of Ficus Racemosa sanctum in deionised water. This extract added to 0.02 M of copper sulfate solution and we observed the change in color of the solution from colorless to colored solution, this indicates that there is a formation of Cu nanoparticles. These biosynthesized Cu nanoparticles were characterized with the help of UV -Visible Spectroscopy. It was observed that the Ficus Racemosa leaf extract can reduce copper ions into copper nanoparticles within 8 to 10 min of reaction time. Thus, this method can be used for rapid and ecofriendly biosynthesis of stable copper nanoparticles. Further, microscopic examination and antimicrobial activity of synthesized nanoparticles was done against several bacteria and fungi.